Team:Warsaw/Calendar-Main/29 May 2008

From 2008.igem.org

(Difference between revisions)
 
(15 intermediate revisions not shown)
Line 1: Line 1:
{{WarNotebook}}
{{WarNotebook}}
<!-- do not edit above me! -->
<!-- do not edit above me! -->
 +
<html>
 +
<h3>Rifampicin test #3</h3>
 +
<h4>Michał L., Ewa, Marcin, Piotr</h4>
 +
<p><ol>
 +
<li> Added 50 μL <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPM-T5+AID</a> (in liquid LB) into liquid LB.</li>
 +
<li> Added 50 μL <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPM-T5+AID</a> (in liquid LB) into liquid LB + 0.02% arabinose.</li>
 +
<li> Added 50 μL <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A+AID</a> (in liquid LB) into liquid LB.</li>
-
1. Add 50 μL E.coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB
+
<li> Added 50 μL <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A+AID</a> (in liquid LB) into liquid LB + 1 mM IPTG.</li>
-
2. Add 50 μL E.coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB + 0.02% arabinose
+
<li> Cultures were incubated for 5 hours.</li>
-
3. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB
 
-
4. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB + 1mM IPTG
+
<li> 1 ml (2x) from p.1, p.2, p.3, p.4 were plated on LB + rifampicin. </li>
-
 
+
-
5. Wait for 5 hours
+
-
 
+
-
6. OD measurement
+
-
 
+
-
7. 1 ml (2x) from p.1, p.2, p.3, p.4 (LB + rifampicin)
+
 +
<li>OD measurement of above cultures.</li>
 +
</ol></p>
 +
</html>
<!-- do not remove this! -->
<!-- do not remove this! -->
{{WarNotebookEnd}}
{{WarNotebookEnd}}

Latest revision as of 14:12, 26 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Rifampicin test #3

Michał L., Ewa, Marcin, Piotr

  1. Added 50 μL E. coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB.
  2. Added 50 μL E. coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB + 0.02% arabinose.
  3. Added 50 μL E. coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB.
  4. Added 50 μL E. coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB + 1 mM IPTG.
  5. Cultures were incubated for 5 hours.
  6. 1 ml (2x) from p.1, p.2, p.3, p.4 were plated on LB + rifampicin.
  7. OD measurement of above cultures.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/29_May_2008"