Team:NTU-Singapore/Notebook/4 June 2008

From 2008.igem.org

(Difference between revisions)
(New page: =Wednesday 4 June= ==Lab details:== *Morning: **Take out 4 cryotubes (2 terminators, 2 LacI-GFP) from yesterday incubation, then spin them down, pour out the liquid to obtain cell pellets....)
 
(3 intermediate revisions not shown)
Line 1: Line 1:
 +
<html><link rel="stylesheet" href="http://greenbear88.googlepages.com/ntu_igem.css" type="text/css"></html>
 +
 +
<div id="header">{{User:Greenbear/sandbox/header}}</div>
 +
 +
<div id="maincontent" style="margin-top:130px;">
 +
<html>
 +
<div id="arrow">
 +
<a href="https://2008.igem.org/Team:NTU-Singapore/Notebook">
 +
<img src="https://static.igem.org/mediawiki/2008/8/8d/Back_to_notebook.png
 +
"
 +
    alt="Back to Notebook"
 +
    title="Back to Notebook">
 +
</a>
 +
</div>
 +
</html>
 +
=Wednesday 4 June=
=Wednesday 4 June=
 +
<div class="quote" style="margin:20px;">
 +
{|border="1" style="background-color:#ffffcc;" cellpadding="20"
 +
|
==Lab details:==
==Lab details:==
 +
===Personnel===
 +
Choon Kit, Hung, Darius:
*Morning:
*Morning:
**Take out 4 cryotubes (2 terminators, 2 LacI-GFP) from yesterday incubation, then spin them down, pour out the liquid to obtain cell pellets.
**Take out 4 cryotubes (2 terminators, 2 LacI-GFP) from yesterday incubation, then spin them down, pour out the liquid to obtain cell pellets.
Line 6: Line 27:
**# 2 for terminators
**# 2 for terminators
**# 2 for LacI-GFP
**# 2 for LacI-GFP
-
**# 1 for CoE7 plasmid from BW strain (from -80 oC fridge)
+
**# 1 for CoE7 plasmid from BW strain (from -20 oC fridge)
-
**# 1 for CoE7 plasmid from W3110 strain (from -80 oC fridge)
+
**# 1 for CoE7 plasmid from W3110 strain (from -20 oC fridge)
*Afternoon:
*Afternoon:
Line 16: Line 37:
**Run gel for morning samples.
**Run gel for morning samples.
**Inoculation of BW (2x),W3110 (2x), MG1655 (2x) for glycerol stock on Thursday.
**Inoculation of BW (2x),W3110 (2x), MG1655 (2x) for glycerol stock on Thursday.
 +
**Prepare LB broth
 +
**Prepare 17x LBA agar plates
 +
</div>

Latest revision as of 00:28, 28 October 2008

Wednesday 4 June

Lab details:

Personnel

Choon Kit, Hung, Darius:

  • Morning:
    • Take out 4 cryotubes (2 terminators, 2 LacI-GFP) from yesterday incubation, then spin them down, pour out the liquid to obtain cell pellets.
    • Prepare 6 digestion solutions for gel electrophoresis in the afternoon:
      1. 2 for terminators
      2. 2 for LacI-GFP
      3. 1 for CoE7 plasmid from BW strain (from -20 oC fridge)
      4. 1 for CoE7 plasmid from W3110 strain (from -20 oC fridge)
  • Afternoon:
    • Amplification for the following plasmids (from Registry):
      1. GFP generator [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0240 BBa_E0240]
      2. RBS [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0032 BBa_B0032] and RBS [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034]
      3. T7 promoter [http://partsregistry.org/wiki/index.php?title=Part:BBa_I719005 BBa_I719005]
    • Run gel for morning samples.
    • Inoculation of BW (2x),W3110 (2x), MG1655 (2x) for glycerol stock on Thursday.
    • Prepare LB broth
    • Prepare 17x LBA agar plates