Team:Warsaw/Calendar-Main/18 July 2008

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<p>'''Cloning of protein A DNA to OmpA constructs'''<br>
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<h3>Cloning of protein Z DNA to <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b-OmpA-omega</a> in place of OmpA</h3><h4>Paweł</h4>
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1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega and pACYC177+OmpA_A_alpha <br>
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<p>Ligation <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">transformed</a> into <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain and plated on LB + ampicillin.</p>
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<h3>Cloning of protein Z DNA to OmpA constructs</h3>
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<h4>Michał K.</h4>
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<p>Colonies from transformation <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a> were inoculated to liquid LB broth with kanamycin. </p>
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Latest revision as of 09:52, 14 October 2008

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Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA

Paweł

Ligation transformed into TOP10 strain and plated on LB + ampicillin.

Cloning of protein Z DNA to OmpA constructs

Michał K.

Colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin.


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