Imperial College/26 September 2008
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*To further check integration, and to test for copying errors during PCR the midipreps that will be taken from these minis will require sequencing | *To further check integration, and to test for copying errors during PCR the midipreps that will be taken from these minis will require sequencing | ||
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+ | ===Single Colony PCR=== | ||
+ | |||
+ | *To confirm successful ligations of our parts into biobrick vectors we carried out single colony PCR (SCP). The gel below shows the results from the experiement. The lane notation is as follows: | ||
+ | **6L = LipA-HE | ||
+ | **LacI - LacI gene | ||
+ | **12 = Pveg-spoVG | ||
+ | **Pos = positive control | ||
+ | **Neg = negative control | ||
+ | |||
+ | [[Image:SCP26.PNG|center|600px]] | ||
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+ | *The results show that LacI<sub> 1 and 2 </sub> have worked and 12 <sub>3</sub> have successfully ligated. None of the 6L constructs have successfully ligated. | ||
+ | <br> | ||
+ | {{Imperial/EndPage|Notebook|Notebook}} |
Latest revision as of 20:49, 28 October 2008
26 September 2008Wet LabCloning
Single Colony PCR
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