Team:Chiba/jk/γ/week 3

From 2008.igem.org

(Difference between revisions)

Latest revision as of 06:30, 24 October 2008

実験ログ

出力班

WEEK 3

31,August,2008

Digestion

[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]①
PCR product([http://partsregistry.org/Part:BBa_J52008 BBa_J52008])②

Sample No.	①	②
DNA	10	10
SpeⅠ	1	0
PstⅠ	1	1
XbaⅠ	0	1
BSA(x100)	0.5	0.5
Buffer2	5	0
Buffer3	0	5
dH₂O	28.5	28.5
TOTAL	50	50

-->37℃ 3hour

-->①20μl refine

-->②20μl refine

1,September,2008

Ligation

vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]①
negative control:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]②

Sample No.	①	②
vector	2	2
insert	3	0
Ligase Buffer	2	2
Ligase	1	1
dH₂O	3	5
TOTAL	10	10

-->R/T 2hour

Transformation

[http://partsregistry.org/Part:BBa_R0079 BBa_R0079](Las promoter)
[http://partsregistry.org/Part:BBa_R0071 BBa_R0071](RhlR promoter)
[http://partsregistry.org/Part:BBa_R0077 BBa_R0077](cinR promoter+RBS)
[http://partsregistry.org/Part:BBa_R0078 BBa_R0078](cinR promoter)
[http://partsregistry.org/Part:BBa_R0062 BBa_R0062](Lux promoter)

2,September,2008

TIME RESPONCE (Solid)

Colony PCR

[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]+[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]

Sample No.	①
culture	1
Fwd primer	1.5
Rev primer	1.5
Thermo pol Buffer	3
dNTP mix	3
Taq DNA pol (NEB)	0.2 (1 unit)
dH₂O	19.8
TOTAL	30

Mini prep

[http://partsregistry.org/Part:BBa_R0079 BBa_R0079]
[http://partsregistry.org/Part:BBa_R0071 BBa_R0071]
[http://partsregistry.org/Part:BBa_R0077 BBa_R0077]
[http://partsregistry.org/Part:BBa_R0078 BBa_R0078]
[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]

3,September,2008

Digestion test

[http://partsregistry.org/Part:BBa_R0079 BBa_R0079]①
[http://partsregistry.org/Part:BBa_R0071 BBa_R0071]②
[http://partsregistry.org/Part:BBa_R0077 BBa_R0077]③
[http://partsregistry.org/Part:BBa_R0078 BBa_R0078]④
[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]⑤

Sample No.	①~⑤
DNA tamplate	5
Buffer3	1
dH₂O	4
EcoRⅠ	0.1
TOTAL	10

-->37℃30min

-->Gel check

Sample No.	①~⑤
DNA(digestion sample)	10
loading Dye	2
TOTAL	12

4,Septempber,2008

Transformation

Ligation product
rLUciferase([http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] ) x3
Venus YFP([http://partsregistry.org/Part:BBa_E2030 BBa_E2030]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]) x3

5,September,2008

TIME RESPONCE(liquid)

Digestion test

[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x2
[http://partsregistry.org/Part:BBa_E2030 BBa_E2030]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x2

DNA(digested sample)	2
PstⅠ	0.1
BSA	0.8
Buffer3	1
dH₂O	7
TOTAL	10

-->37℃ 30in

Gel Check

[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x2①
[http://partsregistry.org/Part:BBa_E2030 BBa_E2030]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x2②
[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](PCR Product)③
[http://partsregistry.org/Part:BBa_E2050 BBa_E2050]④

Sample No.	①~②	③,④
DNA(digested sample)	10	1
loading Dye	2	1
TOTAL	12	6

Luciferase check-->no light

>Last Week

>Next Week

ホーム	メンバー紹介	プロジェクト紹介	Parts Submitted to the Registry	モデリング	ノート

@@ Line 1: / Line 1: @@
-<br>
 [[Team:Chiba/jk|実験ログ]]
-<br>[[Team:Chiba/jk/γ|出力班]]
+[[Team:Chiba/jk/γ|出力班]]
 == WEEK 3 ==
@@ Line 9: / Line 9: @@
 == 31,August,2008 ==
-<br>digestion
+[[Team:Chiba/protocol/digestion|Digestion]]
-<br>BBa_R0010①
-<br>PCR product(BBa_J52008)②
+*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]①
+*PCR product([http://partsregistry.org/Part:BBa_J52008 BBa_J52008])②
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①</td><td>②</td>
 </tr>
@@ Line 46: / Line 48: @@
 </tr>
 <tr>
-<td>dH20</td>
+<td>dH<sub>2</sub>O</td>
 <td>28.5</td><td>28.5</td>
 </tr>
@@ Line 54: / Line 56: @@
 </tr>
 </table>
-<br>->37℃ 3hour
-<br>->①20μl refine
+-->37℃ 3hour
-<br>->②20μl refine
+-->①20μl refine
+-->②20μl refine
 == 1,September,2008 ==
-<br>Ligation
+[[Team:Chiba/protocol/ligation/ligation|Ligation]]
-<br>vector:BBa_R0010 insert:BBa_J52008①
+*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]①
-<br>negative control:BBa_R0010②
+*negative control:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]②
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①</td><td>②</td>
 </tr>
@@ Line 86: / Line 92: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>3</td><td>5</td>
 </tr>
+<tr>
 <td>TOTAL</td>
 <td>10</td><td>10</td>
 </tr>
 </table>
-<br>->R/T 2hour
-<br>TRANSFORMATION
+-->R/T 2hour
-<br>BBa_R0079(Las promoter)
-<br>BBa_R0071(RhlR promoter)
-<br>BBa_R0077(cinR promoter+RBS)
-<br>BBa_R0078(cnR promoter)
-<br>BBa_R0062(LUX promoter)
+[[Team:Chiba/protocol/transformation|Transformation]]
+*[http://partsregistry.org/Part:BBa_R0079 BBa_R0079](Las promoter)
+*[http://partsregistry.org/Part:BBa_R0071 BBa_R0071](RhlR promoter)
+*[http://partsregistry.org/Part:BBa_R0077 BBa_R0077](cinR promoter+RBS)
+*[http://partsregistry.org/Part:BBa_R0078 BBa_R0078](cinR promoter)
+*[http://partsregistry.org/Part:BBa_R0062 BBa_R0062](Lux promoter)
@@ Line 107: / Line 115: @@
 == 2,September,2008 ==
-<br>[[Team:Chiba/jk/γ/trs|TIME RESPONCE (Solid)]]
+[[Team:Chiba/jk/γ/trs|TIME RESPONCE (Solid)]]
+Colony PCR
+*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]+[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]
-<br>COLONY PCR
-<br>BBa_R0010+BBa_J52008
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①</td>
 </tr>
@@ Line 120: / Line 129: @@
 <td>1</td>
 </tr>
+<tr>
 <td>Fwd primer</td>
 <td>1.5</td>
@@ Line 140: / Line 150: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>19.8</td>
 </tr>
+<tr>
 <td>TOTAL</td>
 <td>30</td>
 </tr>
 </table>
-<br>MINI Prep
-<br>BBa_R0079
+[[Team:Chiba/protocol/DNA_Purification/sigma|Mini prep]]
-<br>BBa_R0071
+*[http://partsregistry.org/Part:BBa_R0079 BBa_R0079]
-<br>BBa_R0077
+*[http://partsregistry.org/Part:BBa_R0071 BBa_R0071]
-<br>BBa_R0078
+*[http://partsregistry.org/Part:BBa_R0077 BBa_R0077]
-<br>BBa_R0062
+*[http://partsregistry.org/Part:BBa_R0078 BBa_R0078]
+*[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]
 == 3,September,2008 ==
-<br>Digestion test
+[[Team:Chiba/protocol/digestion|Digestion test]]
-<br>BBa_R0079①
+*[http://partsregistry.org/Part:BBa_R0079 BBa_R0079]①
-<br>BBa_R0071②
+*[http://partsregistry.org/Part:BBa_R0071 BBa_R0071]②
-<br>BBa_R0077③
+*[http://partsregistry.org/Part:BBa_R0077 BBa_R0077]③
-<br>BBa_R0078④
+*[http://partsregistry.org/Part:BBa_R0078 BBa_R0078]④
-<br>BBa_R0062⑤
+*[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]⑤
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①~⑤</td>
 </tr>
@@ Line 176: / Line 189: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>4</td>
 </tr>
@@ Line 183: / Line 196: @@
 <td>0.1</td>
 </tr>
+<tr>
 <td>TOTAL</td>
 <td>10</td>
 </tr>
 </table>
-<br>->37℃30min
-<br>->Gel cheack
+-->37℃30min
+-->[[Team:Chiba/protocol/gelcheck|Gel check]]
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①~⑤</td>
 </tr>
@@ Line 208: / Line 225: @@
 </table>
+== 4,Septempber,2008 ==
+[[Team:Chiba/protocol/transformation|Transformation]]
-== 4,Septempber,2008 ==
+*Ligation product
-<br>TANSFORMATION
+*rLUciferase([http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] )   x3
-<br>LIgation product
+*Venus YFP([http://partsregistry.org/Part:BBa_E2030 BBa_E2030]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620])    x3
-<br>rLUciferase(BBa_J52008+BBa_F2620 )   x3
-<br>Venus YFP(BBa_E2030+BBa_F2620)    x3
 == 5,September,2008 ==
-<br>[[Team:Chiba/jk/γ/Trl|TIME RESPONCE(liquid)]]
+[[Team:Chiba/jk/γ/Trl|TIME RESPONCE(liquid)]]
-<br>Digestion test
-<br>BBa_J52008+BBa_F2620 x2
+[[Team:Chiba/protocol/digestion|Digestion test]]
-<br>BBa_E2030+BBa_F2620  x2
+*[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x2
+*[http://partsregistry.org/Part:BBa_E2030 BBa_E2030]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]  x2
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">DNA(digestion sample)</td>
+<td width="257">DNA(digested sample)</td>
 <td>2</td>
 </tr>
@@ Line 241: / Line 259: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>7</td>
 </tr>
@@ Line 250: / Line 268: @@
 </table>
-<br>--->37℃ 30in
+-->37℃ 30in
-<br>Gel Cheack
-<br>BBa_J52008+BBa_F2620 x2①
+[[Team:Chiba/protocol/gelcheck|Gel Check]]
-<br>BBa_E2030+BBa_F2620  x2②
+*[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x2①
-<br>BBa_J52008(PCR Product)③
+*[http://partsregistry.org/Part:BBa_E2030 BBa_E2030]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]  x2②
-<br>BBa_E2050④
+*[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](PCR Product)③
+*[http://partsregistry.org/Part:BBa_E2050 BBa_E2050]④
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①~②</td><td>③,④</td>
 </tr>
 <tr>
-<td>DNA(digestion sample)</td>
+<td>DNA(digested sample)</td>
 <td>10</td><td>1</td>
 </tr>
@@ Line 269: / Line 288: @@
 <td>loading Dye</td>
 <td>2</td><td>1</td>
-</>tr>
+</tr>
 <tr>
 <td>TOTAL</td>
-<td>12</td>
+<td>12</td><td>6</td>
 </tr>
 </table>
-<br>Luciferase cheack->no light
+*Luciferase check-->no light
+[[Team:Chiba/jk/γ/week 2|>Last Week]]
-<br>[[Team:Chiba/jk/γ/week 2|>Last Week]]
+[[Team:Chiba/jk/γ/week 4|>Next Week]]
-<br>[[Team:Chiba/jk/γ/week 4|>Next Week]]

Team:Chiba/jk/γ/week 3

From 2008.igem.org

Latest revision as of 06:30, 24 October 2008

Contents

WEEK 3

31,August,2008

1,September,2008

2,September,2008

3,September,2008

4,Septempber,2008

5,September,2008