Team:Warsaw/Calendar-Main/25 July 2008
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MKrzyszton (Talk | contribs) (New page: {{WarNotebook}} <!-- do not edit above me! --> <p>1. Isolation of plasmids from cultures inocluated on previous day.<br> 2. Control digestion of isolated plasmids with BamHI and SacI (...) |
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- | <p> | + | <h3>Cloning of protein Z DNA to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b-OmpA-omega</a> in place of OmpA</h3> |
- | + | <h4>Paweł</h4> | |
- | </p> | + | <p><ol> |
+ | <li><a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation>Isolation</a> of plasmids from cultures inoculated on previous day.</li> | ||
+ | <li>Control <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest>digest</a> of isolated plasmids with NdeI and NotI. Two positives obtained (proper band: ~200 bp visible).</li> | ||
+ | <li>One of positive plasmids transformed into <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> and plated on LB+amp, overnight, for further isolation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2Bhis%2BZ%2Bomega>pET15b+Z+omega</a> vector.</li> | ||
+ | </ol></p> | ||
+ | <h3>Cloning of protein A DNA to OmpA constructs</h3><h4>Michał K.</h4> | ||
+ | <ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>).</li> | ||
+ | <li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI (BamHI buffer).</li><li> Gel electrophoresis - proper clone found. </li></ol> | ||
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Latest revision as of 14:52, 25 October 2008
Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpAPaweł
Cloning of protein A DNA to OmpA constructsMichał K.
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