Team:Warsaw/Calendar-Main/17 July 2008
From 2008.igem.org
(Difference between revisions)
(21 intermediate revisions not shown) | |||
Line 2: | Line 2: | ||
<!-- do not edit above me! --> | <!-- do not edit above me! --> | ||
+ | <html> | ||
+ | <h3>Cloning of protein Z DNA to OmpA constructs</h3><h4>Michał K.</h4> | ||
+ | <p><ol> | ||
+ | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation (with CIAP)</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> with SacI and NotI (BamHI buffer). </li> | ||
+ | <li> Gel eloctrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> (4300 bp band). </li> | ||
+ | <li> | ||
+ | <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragment and Z DNA fragment isolated on <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/14_July_2008">14 July</a>. </li> | ||
+ | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with ligation. </li> | ||
+ | <li> Transformants plating on LB + kanamycin. </li> | ||
+ | </ol></p> | ||
+ | <br> | ||
+ | <br> | ||
- | < | + | <h3>Cloning of protein Z DNA to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b-OmpA-omega</a> in place of OmpA</h3><h4>Paweł</h4> |
- | + | <p><ol><li><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b-OmpA-omega</a> and Z (in <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pGeneart_Z>Geneart vector</a>) with NdeI and NotI (Orange buffer).</li> | |
- | + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Gel-out</a> of Z (~200 bp band).</li> | |
- | + | <li>Overnight <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">ligation</a> of Z into digested <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b-OmpA-omega</a>.</li> | |
- | + | ||
- | + | ||
- | </ | + | |
- | |||
+ | </ol></p> | ||
+ | |||
+ | |||
+ | </html> | ||
<!-- do not remove this! --> | <!-- do not remove this! --> | ||
{{WarNotebookEnd}} | {{WarNotebookEnd}} |
Latest revision as of 09:10, 14 October 2008
Cloning of protein Z DNA to OmpA constructsMichał K.
Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpAPaweł
|