Team:Warsaw/Calendar-Main/10 June 2008

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<h3>Michał L., Ewa, Marcin</h3>
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<html><h3>Design of primers for fusion of protein A, OmpA and alpha and omega fragments of <br>beta-lactamase </h3><h4>Piotr</h4>
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<p><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Plasmid isolation</a> from overnight culture of our transformants.<br>
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<p><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaL_link">alphaL+link14+homo (alphaL_link)</a>
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<br>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">alphaP+STOP+XbaI+BamHI (alphaP_XB)</a><br>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL_link">omegaL+link14+homo (omegaL_link)</a><br>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">omegaP+STOP+EcoRI+PstI+BamHI (omegaP_EPB)</a><br>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpAL+NdeI (ompaL_N)</a> <br>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpAP+link8+SacI+NotI+homo (ompaP_link)</p>
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Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.<br>
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Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI.<br>
 
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Control digest of pMPM-T5 + T7 RNA-polymerase with EcoRI.<br>
 
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Control digest of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI.<br>
 
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DNA gel electrophoresis of digestion products.<br>
 
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Picking out proper colonies of transformants.<br>
 
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</p>
 

Latest revision as of 16:59, 12 October 2008

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Design of primers for fusion of protein A, OmpA and alpha and omega fragments of
beta-lactamase

Piotr

alphaL+link14+homo (alphaL_link)
alphaP+STOP+XbaI+BamHI (alphaP_XB)
omegaL+link14+homo (omegaL_link)
omegaP+STOP+EcoRI+PstI+BamHI (omegaP_EPB)
OmpAL+NdeI (ompaL_N)
OmpAP+link8+SacI+NotI+homo (ompaP_link)

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