Template:Team:UC Berkeley/Notebook/BX construction

From 2008.igem.org

(Difference between revisions)
 
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__TOC__
__TOC__
-
==K112000==
+
==pBca1256-K112000==
<pre>
<pre>
PCR bx1/bx2 on Bjh1301 (688 bp, EcoRI/BamHI/DpnI)
PCR bx1/bx2 on Bjh1301 (688 bp, EcoRI/BamHI/DpnI)
Line 13: Line 13:
</pre>
</pre>
-
==K112001==
+
==pBca1256-K112001==
<pre>
<pre>
PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
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</pre>
</pre>
-
==K112002==
+
==pBca1256-K112002==
<pre>
<pre>
PCR bx1/bx4 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
PCR bx1/bx4 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
Line 37: Line 37:
</pre>
</pre>
-
==K112003==
+
==pBca1256-K112003==
<pre>
<pre>
PCR bx3/bx4 on Bjh1301 (682 bp, EcoRI/BamHI/DpnI)
PCR bx3/bx4 on Bjh1301 (682 bp, EcoRI/BamHI/DpnI)
Line 49: Line 49:
</pre>
</pre>
-
==K112004==
+
==pBca1256-K112004==
<pre>
<pre>
PCR bx5/bx2 on Bjh1301 (692 bp, EcoRI/BamHI/DpnI)
PCR bx5/bx2 on Bjh1301 (692 bp, EcoRI/BamHI/DpnI)
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</pre>
</pre>
-
==K112005==
+
==pBca1256-K112005==
<pre>
<pre>
PCR bx6/bx2 on Bjh1301 (704 bp, EcoRI/BamHI/DpnI)
PCR bx6/bx2 on Bjh1301 (704 bp, EcoRI/BamHI/DpnI)
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</pre>
</pre>
-
==K112006==
+
==pBca1256-K112006==
<pre>
<pre>
PCR bx7/bx8 on Bjh1303 (325 bp, EcoRI/BamHI/DpnI)
PCR bx7/bx8 on Bjh1303 (325 bp, EcoRI/BamHI/DpnI)
Line 85: Line 85:
</pre>
</pre>
-
==K112007==
+
==pBca1256-K112007==
<pre>
<pre>
PCR bx9/bx8 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI)
PCR bx9/bx8 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI)
Line 97: Line 97:
</pre>
</pre>
 +
==pBca1256-K112008==
<pre>
<pre>
PCR bx7/bx10 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI)
PCR bx7/bx10 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI)
Line 108: Line 109:
</pre>
</pre>
 +
==pBca1256-K112009==
<pre>
<pre>
PCR bx9/bx10 on Bjh1303 (319 bp, EcoRI/BamHI/DpnI)
PCR bx9/bx10 on Bjh1303 (319 bp, EcoRI/BamHI/DpnI)
Line 119: Line 121:
</pre>
</pre>
 +
==pBca1256-K112010==
<pre>
<pre>
PCR bx11/bx8 on Bjh1303 (329 bp, EcoRI/BamHI/DpnI)
PCR bx11/bx8 on Bjh1303 (329 bp, EcoRI/BamHI/DpnI)
Line 130: Line 133:
</pre>
</pre>
 +
==pBca1256-K112011==
<pre>
<pre>
PCR bx12/bx8 on Bjh1303 (339 bp, EcoRI/BamHI/DpnI)
PCR bx12/bx8 on Bjh1303 (339 bp, EcoRI/BamHI/DpnI)
Line 141: Line 145:
</pre>
</pre>
 +
==pBca1256-K112012==
<pre>
<pre>
PCR bx13/bx14 on Bjh1302 (526 bp, EcoRI/BamHI/DpnI)
PCR bx13/bx14 on Bjh1302 (526 bp, EcoRI/BamHI/DpnI)
Line 152: Line 157:
</pre>
</pre>
 +
==pBca1256-K112013==
<pre>
<pre>
PCR bx15/bx14 on Bjh1302 (523 bp, EcoRI/BamHI/DpnI)
PCR bx15/bx14 on Bjh1302 (523 bp, EcoRI/BamHI/DpnI)
Line 163: Line 169:
</pre>
</pre>
 +
==pBca1256-K112014==
<pre>
<pre>
PCR bx13/bx16 on Bjh1302 (523 bp, EcoRI/BamHI/DpnI)
PCR bx13/bx16 on Bjh1302 (523 bp, EcoRI/BamHI/DpnI)
Line 174: Line 181:
</pre>
</pre>
 +
==pBca1256-K112015==
<pre>
<pre>
PCR bx15/bx16 on Bjh1302 (520 bp, EcoRI/BamHI/DpnI)
PCR bx15/bx16 on Bjh1302 (520 bp, EcoRI/BamHI/DpnI)
Line 185: Line 193:
</pre>
</pre>
 +
==pBca1256-K112016==
<pre>
<pre>
PCR bx17/bx16 on Bjh1302 (530 bp, EcoRI/BamHI/DpnI)
PCR bx17/bx16 on Bjh1302 (530 bp, EcoRI/BamHI/DpnI)
Line 196: Line 205:
</pre>
</pre>
 +
==pBca1256-K112017==
<pre>
<pre>
PCR bx18/bx16 on Bjh1302 (542 bp, EcoRI/BamHI/DpnI)
PCR bx18/bx16 on Bjh1302 (542 bp, EcoRI/BamHI/DpnI)

Latest revision as of 01:36, 12 June 2008

Contents


pBca1256-K112000

PCR bx1/bx2 on Bjh1301		(688 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112000
----------------------------------------
bx1		Forward Biobricking of {T4 Holin!}
ccagtGAATTCatgAGATCTatggcagcacctagaatatc
bx2		Reverse Biobricking of {T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

pBca1256-K112001

PCR bx3/bx2 on Bjh1301		(685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112001
----------------------------------------
bx3		Forward biobricking of {<T4 Holin!}
ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
bx2		Reverse biobricking of {<T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

pBca1256-K112002

PCR bx1/bx4 on Bjh1301		(685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112002
----------------------------------------
bx1		Forward biobricking of {T4 Holin>}
ccagtGAATTCatgAGATCTatggcagcacctagaatatc
bx4		Reverse biobricking of {T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc

pBca1256-K112003

PCR bx3/bx4 on Bjh1301		(682 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112003
----------------------------------------
bx3		Forward Biobricking of {<T4 Holin>}
ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
bx4		Reverse Biobricking of {<T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc

pBca1256-K112004

PCR bx5/bx2 on Bjh1301		(692 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112004
----------------------------------------
bx5		Forward Biobricking of {a~T4 Holin!}
ccagtGAATTCatgAGATCTgtctatggcagcacctagaatatc
bx2		Reverse Biobricking of {a~T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

pBca1256-K112005

PCR bx6/bx2 on Bjh1301		(704 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112005
----------------------------------------
bx6		Forward Biobricking of {rbs.T4 Holin!}
ccagtGAATTCatgAGATCTcttaaaaggagggtctatggcagcacctagaatatc
bx2		Reverse Biobricking of {rbs.T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc

pBca1256-K112006

PCR bx7/bx8 on Bjh1303		(325 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112006
----------------------------------------
bx7		Forward biobricking of {T4 Antiholin!}
ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac
bx8		Reverse biobricking of {T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc

pBca1256-K112007

PCR bx9/bx8 on Bjh1303		(322 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112007
----------------------------------------
bx9		Forward biobricking of {<T4 Antiholin!}
ccagtGAATTCatgAGATCTgccttaaaagcaacagcac
bx8		Reverse biobricking of {<T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc

pBca1256-K112008

PCR bx7/bx10 on Bjh1303		(322 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112008
----------------------------------------
bx7		Forward biobricking of {T4 Antiholin>}
ccagtGAATTCatgAGATCTatggccttaaaagcaacagcac
bx10	Reverse biobricking of {T4 Antiholin>}
cgttaGGATCCttcagtctccaatttaatgttc

pBca1256-K112009

PCR bx9/bx10 on Bjh1303		(319 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112009
----------------------------------------
bx9		Forward biobricking of {<T4 Antiholin>}
ccagtGAATTCatgAGATCTgccttaaaagcaacagcac
bx10	Reverse biobricking of {<T4 Antiholin>}
cgttaGGATCCttcagtctccaatttaatgttc

pBca1256-K112010

PCR bx11/bx8 on Bjh1303		(329 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112010
----------------------------------------
bx11	Forward biobricking of {a~T4 Antiholin!}
ccagtGAATTCatgAGATCTctttatggccttaaaagcaacagc
bx8		Reverse biobricking of {a~T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc

pBca1256-K112011

PCR bx12/bx8 on Bjh1303		(339 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112011
----------------------------------------
bx12	Forward biobricking of {rbs.T4 Antiholin!}
ccagtGAATTCatgAGATCTgataggaggcctttatggccttaaaagcaacagc
bx8		Reverse biobricking of {rbs.T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc

pBca1256-K112012

PCR bx13/bx14 on Bjh1302		(526 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112012
----------------------------------------
bx13      Forward biobricking of {T4 Endolysin!}
ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg
bx14      Reverse biobricking of {T4 Endolysin!}
cgttaGGATCCttatagatttttatacgcgtcccaagtgcc

pBca1256-K112013

PCR bx15/bx14 on Bjh1302		(523 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112013
----------------------------------------
bx15      Forward biobricking of {<T4 Endolysin!}
ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg
bx14      Reverse biobricking of {<T4 Endolysin!}
cgttaGGATCCttatagatttttatacgcgtcccaagtgcc

pBca1256-K112014

PCR bx13/bx16 on Bjh1302		(523 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112014
----------------------------------------
bx13      Forward biobricking of {T4 Endolysin>}
ccagtGAATTCatgAGATCTatgaatatatttgaaatgttacg
bx16      Reverse biobricking of {T4 Endolysin>}
cgttaGGATCCtagatttttatacgcgtcccaagtgcc

pBca1256-K112015

PCR bx15/bx16 on Bjh1302		(520 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112015
----------------------------------------
bx15      Forward biobricking of {<T4 Endolysin>}
ccagtGAATTCatgAGATCTaatatatttgaaatgttacgtatagatg
bx16      Reverse biobricking of {<T4 Endolysin>}
cgttaGGATCCtagatttttatacgcgtcccaagtgcc

pBca1256-K112016

PCR bx17/bx16 on Bjh1302		(530 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112016
----------------------------------------
bx17      Forward biobricking of {a~T4 Endolysin!}
ccagtGAATTCatgAGATCTtattatgaatatatttgaaatgttacgtatagatg
bx16      Reverse biobricking of {a~T4 Endolysin!}
cgttaGGATCCtagatttttatacgcgtcccaagtgcc

pBca1256-K112017

PCR bx18/bx16 on Bjh1302		(542 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256				(EcoRI/BamHI)
Product is pBca1256-K112017
----------------------------------------
bx18      Forward biobricking of {rbs.T4 Endolysin!}
ccagtGAATTCatgAGATCTatacttaggaggtattatgaatatatttgaaatgttac
bx16      Reverse biobricking of {rbs.T4 Endolysin!}
cgttaGGATCCtagatttttatacgcgtcccaagtgcc



Bing Xia
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