Team:Warsaw/Calendar-Main/5 September 2008

From 2008.igem.org

(Difference between revisions)
(New page: {{WarNotebook}} <!-- do not edit above me! --> Oczyszczanie białek: A-alfa, Z-alfa i Z-omega Piotr Zwirowanie bakterii z 1l, sonikacja ich (protokół) zwirowanie sonikatu w przypa...)
 
(6 intermediate revisions not shown)
Line 2: Line 2:
<!-- do not edit above me! -->
<!-- do not edit above me! -->
 +
<html>
 +
<h3>Purification of proteins: A-alpha</h3><h4>Piotr, Emilia</h4>
 +
<p><ol><li>Bacteria were centrifuged and sonicated (according to <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o>protocol</a>).</li>
 +
<li>Sonicate was centrifuged. </li>
 +
<li>Pellet was discarded, supernatant was kept for further purification steps.</li></ol></p>
 +
<br>
-
 
+
</html>
-
Oczyszczanie białek: A-alfa, Z-alfa i Z-omega
+
-
 
+
-
Piotr
+
-
Zwirowanie bakterii z 1l, sonikacja ich (protokół)
+
-
zwirowanie sonikatu
+
-
w przypadku z-alfa i z-omega odżucenie supernatantu, zawieszenie osadu w wodzi i ponowne zwirowanie i odżucenie wody i zawieszenie w wodzie
+
-
w przypadku a-alfa zatrzymanie supernatantu odżucenie osadu
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
<!-- do not remove this! -->
<!-- do not remove this! -->
{{WarNotebookEnd}}
{{WarNotebookEnd}}

Latest revision as of 21:06, 26 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Purification of proteins: A-alpha

Piotr, Emilia

  1. Bacteria were centrifuged and sonicated (according to protocol).
  2. Sonicate was centrifuged.
  3. Pellet was discarded, supernatant was kept for further purification steps.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/5_September_2008"