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Team:UNIPV-Pavia/Protocols/GelExtraction
From 2008.igem.org
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| - | [https://www.roche-applied-science.com/pack-insert/1696505a.pdf] | + | {| style="color:#000000;background-color:#ffffff;" cellpadding="3" cellspacing="1" border="3" bordercolor="#3128" width="80%" align="center" |
| + | !align="center"|[[Image:home.jpg|30px]] [[Team:UNIPV-Pavia|Home]] | ||
| + | !align="center"|[[Image:unipv_logo.jpg|30px]] [[Team:UNIPV-Pavia/Team|The Team]] | ||
| + | !align="center"|[[Image:and.jpg|30px]] [[Team:UNIPV-Pavia/Project|The Project]] | ||
| + | !align="center"|[[Image:safety.jpg|30px]] [[Team:UNIPV-Pavia/Safety|Biological Safety]] | ||
| + | !align="center"|[[Image:dna.png|30px]] [[Team:UNIPV-Pavia/Parts|Parts Submitted to the Registry]] | ||
| + | |- | ||
| + | !align="center"|[[Image:laptop.jpg|30px]] [[Team:UNIPV-Pavia/Dry_Lab|Dry Lab]] | ||
| + | !align="center"|[[Image:pipette.jpg|30px]] [[Team:UNIPV-Pavia/Wet_Lab|Wet Lab]] | ||
| + | !align="center"|[[Image:math.gif|30px]] [[Team:UNIPV-Pavia/Modeling|Modeling]] | ||
| + | !align="center"|[[Image:note.jpg|30px]] [[Team:UNIPV-Pavia/Protocols|Protocols]] | ||
| + | !align="center"|[[Image:notebook.gif|30px]] [[Team:UNIPV-Pavia/Notebook|Activity Notebook]] | ||
| + | |} | ||
| + | |||
| + | <br> | ||
| + | |||
| + | <h1>The protocols we used</h1> | ||
| + | |||
| + | *[[Team:UNIPV-Pavia/Protocols/Lb|LB medium preparation]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/Resuspension|Plasmid resuspension from IGEM paper spots]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/Transformation|Transformation]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/PlasmidExtraction|Plasmid extraction]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/Digestion|BioBrick digestion with restriction enzymes]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/GelExtraction|DNA gel extraction]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/Precipitation|DNA precipitation with sodium acetate]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/AntarcticPhosphatase|Antarctic Phosphatase]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/Ligation|Ligation]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/Pcr|PCR]] | ||
| + | |||
| + | |||
| + | <h1>DNA gel extraction</h1> | ||
| + | ''(Estimated time: 1 hour and 30 min)'' | ||
| + | <br> | ||
| + | <br> | ||
| + | '''Materials needed:''' | ||
| + | *'''Agarose gel with well-separated, sharp bands''' | ||
| + | *'''Scalpel and tweezers to cut gel''' (they have to be cleaned in ddH2O after every cut) | ||
| + | *'''Roche Agarose Gel DNA Extraction Kit''' | ||
| + | <br> | ||
| + | *Follow Roche standard protocol: [https://www.roche-applied-science.com/pack-insert/1696505a.pdf Roche Agarose Gel DNA Extraction Kit protocol] | ||
| + | <br> | ||
Latest revision as of 12:52, 1 July 2008
 Home
|  The Team
|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
The protocols we used
- LB medium preparation
- Plasmid resuspension from IGEM paper spots
- Transformation
- Plasmid extraction
- BioBrick digestion with restriction enzymes
- DNA gel extraction
- DNA precipitation with sodium acetate
- Antarctic Phosphatase
- Ligation
- PCR
DNA gel extraction
(Estimated time: 1 hour and 30 min)
Materials needed:
- Agarose gel with well-separated, sharp bands
- Scalpel and tweezers to cut gel (they have to be cleaned in ddH2O after every cut)
- Roche Agarose Gel DNA Extraction Kit
- Follow Roche standard protocol: Roche Agarose Gel DNA Extraction Kit protocol
