Team:Warsaw/Calendar-Main/5 September 2008

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<h3>Purification of proteins: A-alpha, Z-alpha and Z-omega<br>Piotr</h3>
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<h3>Purification of proteins: A-alpha</h3><h4>Piotr, Emilia</h4>
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<p><ol><li>Bacteria centrifuged and sonicated (according to <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o>protocol</a>).</li>
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<p><ol><li>Bacteria were centrifuged and sonicated (according to <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o>protocol</a>).</li>
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<li>Sonicate centrifuged. </li>
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<li>Sonicate was centrifuged. </li>
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<li>In case of Z-alpha and Z-omega: supernatant discarded, pellet resuspended in ddH20, centrifuged and again resuspended in ddH20</li>
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<li>Pellet was discarded, supernatant was kept for further purification steps.</li></ol></p>
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<li>In case of A-alpha: pellet discarded, supernatant kept for further purification steps</li></ol></p>
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<h3>Protein A mutagenesis<br>Paweł</h3>
 
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<p><ol><li>Treatment of mutageneses as on 2nd September. </li></ol></p>
 
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Purification of proteins: A-alpha

Piotr, Emilia

  1. Bacteria were centrifuged and sonicated (according to protocol).
  2. Sonicate was centrifuged.
  3. Pellet was discarded, supernatant was kept for further purification steps.

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