"
Team:UNIPV-Pavia/Protocols/GelExtraction
From 2008.igem.org
(Difference between revisions)
| (4 intermediate revisions not shown) | |||
| Line 23: | Line 23: | ||
*[[Team:UNIPV-Pavia/Protocols/Digestion|BioBrick digestion with restriction enzymes]] | *[[Team:UNIPV-Pavia/Protocols/Digestion|BioBrick digestion with restriction enzymes]] | ||
*[[Team:UNIPV-Pavia/Protocols/GelExtraction|DNA gel extraction]] | *[[Team:UNIPV-Pavia/Protocols/GelExtraction|DNA gel extraction]] | ||
| + | *[[Team:UNIPV-Pavia/Protocols/Precipitation|DNA precipitation with sodium acetate]] | ||
*[[Team:UNIPV-Pavia/Protocols/AntarcticPhosphatase|Antarctic Phosphatase]] | *[[Team:UNIPV-Pavia/Protocols/AntarcticPhosphatase|Antarctic Phosphatase]] | ||
*[[Team:UNIPV-Pavia/Protocols/Ligation|Ligation]] | *[[Team:UNIPV-Pavia/Protocols/Ligation|Ligation]] | ||
| Line 29: | Line 30: | ||
<h1>DNA gel extraction</h1> | <h1>DNA gel extraction</h1> | ||
| - | ''(Estimated time: 1 hour)'' | + | ''(Estimated time: 1 hour and 30 min)'' |
<br> | <br> | ||
<br> | <br> | ||
'''Materials needed:''' | '''Materials needed:''' | ||
| - | *''' | + | *'''Agarose gel with well-separated, sharp bands''' |
| - | *'''Scalpel and tweezers to cut gel''' | + | *'''Scalpel and tweezers to cut gel''' (they have to be cleaned in ddH2O after every cut) |
*'''Roche Agarose Gel DNA Extraction Kit''' | *'''Roche Agarose Gel DNA Extraction Kit''' | ||
<br> | <br> | ||
*Follow Roche standard protocol: [https://www.roche-applied-science.com/pack-insert/1696505a.pdf Roche Agarose Gel DNA Extraction Kit protocol] | *Follow Roche standard protocol: [https://www.roche-applied-science.com/pack-insert/1696505a.pdf Roche Agarose Gel DNA Extraction Kit protocol] | ||
<br> | <br> | ||
Latest revision as of 12:52, 1 July 2008
 Home
|  The Team
|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
The protocols we used
- LB medium preparation
- Plasmid resuspension from IGEM paper spots
- Transformation
- Plasmid extraction
- BioBrick digestion with restriction enzymes
- DNA gel extraction
- DNA precipitation with sodium acetate
- Antarctic Phosphatase
- Ligation
- PCR
DNA gel extraction
(Estimated time: 1 hour and 30 min)
Materials needed:
- Agarose gel with well-separated, sharp bands
- Scalpel and tweezers to cut gel (they have to be cleaned in ddH2O after every cut)
- Roche Agarose Gel DNA Extraction Kit
- Follow Roche standard protocol: Roche Agarose Gel DNA Extraction Kit protocol
