Edinburgh/Notebook/Ligation products

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Latest revision as of 23:39, 29 October 2008

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Overview Step1 Step2

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Ligation Products

L1: Edinbrick1+dxs (26.08.08)
L2: Edinbrick1+appY (26.08.08)
L3: Babel1+glgC (26.08.08)
L4: Babel2+glgC (26.08.08)
L5: Edinbrick1+appY repeat (initially labelled L4 in error) (04.07.08)
L6: Self ligation of mutagenic PCR P6 (BABEL2+glgC-mut1). (07.07.08)
L7: Self ligation of mutagenic PCR P7 (BABEL2+glgC-mut2). (07.07.08)
L8: Ligation of synthetic ribosome binding site to M2 (pSB1A2+dxs, BBa_K118000). (09.07.08)
L9: Ligation of Edinbrick1 with crtB. (10.07.08)
L10: Ligation of Edinbrick1 with crtI (mutant lacking PstI sites, I hope). (10.07.08)
L11: repeat ligation of synthetic ribosome binding site to M2 (pSB1A2+dxs, BBa_K118000). (11.07.08: Yan, HX)
L12: rbs ligated with pSB1A2+appY (M15). (14.07.08)
L13 and L14: self ligations of P13 and P14 (BABEL2+glgC-mut1,2). (14.07.08)
L15: ligation of Edinbrick1 with rbs+dxs fusion PCR product (P12). (14.07.08) - Failed
L16: Ligation of Edinbrick1 with rbs+appY fusion PCR product (P15) (17.07.08) - Failed
L17: Ligation of rbs to pSB1A2+crtB (M36) (22.07.08: OG, Yan)
L18: Ligation of rbs to pSB1A2+crtI (M42) (22.07.08: OG, Yan)
L19: pSB1A2+rbs+dxs ligation product (From P12) (25.07.08: Yan, OG)
L20: pSB1A2+rbs+appY ligation product (From P15) (25.07.08: Yan, OG)
L21: pSB1A2+rbs+crtB ligation product (From P28) (25.07.08: Yan, OG)
L22: pSB1A2+rbs+crtI ligation product (From P29) (25.07.08: Yan, OG)
L23: pSB1A2+pZntA ligation product (From P31) (25.07.08: Yan, OG)
L24: Ligation of crtE to pSB1A2 (25.07.08: CF)
L25: Ligation of glgC-mut1,2 (P36) to Edinbrick1, (28.07.2008: AM)
L26: Ligation of P15 (rbs+appY) to Edinbrick1. (30.07.2008: HX)
L27: Ligation of P28 (rbs+crtB) to Edinbrick1. (30.07.2008: HX)
L28: Putative cenA ligated to Edinbrick1. (30.07.08: AM)
L29: Putative cex ligated to Edinbrick1. (30.07.08: AM)
L30: Self-ligation of P50 (3rd mutagenesis of glgC-mut1,2) (05.08.08: HX)
L31: Ligation of P36 (glgC-mut1,2, hopefully, could be P39) to Edinbrick1 (05.08.08: OG)
L32: Ligation of rbs+crtB (M67) to rbs+crtI+pSB1A2 (M50) to create pSB1A2+crtB+crtI (07.08.08: AM, Yan)
L33: Ligation of PcstA (P54) to Edinbrick1. (08.08.08: OG)
L34: Ligation of pSB1A2+rbs+dxs with rbs+crtE to make pSB1A2+dxs+crtE. (08.08.08: Yan)
L35: Ligation of pSB1A2+rbs+dxs with rbs+LIMS1 to make pSB1A2+dxs+crtE.(08.08.08: Yan)
L36: pSB1A2+crtY (P59 self-ligation). (09.08.08: CF)
L37: pSB1A2+rbs+crtY (P60 self-ligation). (09.08.08: CF)
L38: crtE+pSB1A2 (M79 into Edinbrick1) (12.08.08: AH)
L39: cex into Edinbrick1 (13.08.08:YAN)
L40: cenA into Edinbrick1 (13.08.08:YAN)
L42: dxs+LIMS (M137) to appY (M82). (18.08.08: YAN)
L43: crtB+crtI (M109) to appY (M82). (18.08.08: YAN)
L44: PcstA (M130) to lacZ. (18.08.08: YAN)
L45: glgC mut 1+2 (M71A) to SOB2 (18.08.08: AM)
L46: glgC mut 1+2+3 (M71) to SOB2 (18.08.08: AM)
L47: glgC mut 1+2+3, a.k.a. glgC16 (M72) to SOB2 (18.08.08: AM)
L48: appY into dxs+LIMS (19.08.08 YAN)
L49: appY into crtBI (19.08.08 YAN)
L50: Self-ligation of P78 (SOB2-glgC mut 1+2) (20.08.08 AM)
L51: Self-ligation of P79 (SOB2-glgC mut 1+2+3) (20.08.08 AM)
L52: Self-ligation of P80 (SOB2-glgC mut 1+2+3) (20.08.08 AM)
L53: Self-ligation of P81 (SOB2-rbs-glgC mut 1+2) (20.08.08 AM)
L54: Self-ligation of P82 (SOB2-rbs-glgC mut 1+2+3) (20.08.08 AM)
L55: Self-ligation of P83 (SOB2-rbs-glgC mut 1+2+3) (20.08.08 AM)
L56: lacZ into PcstA (20.08.08 YAN)
L57: appY into crtBI (28.08.08 YAN)
L58: Self-ligation of P91 (03.09.08: YAN)
L59: Self-ligation of P92 (03.09.08: YAN)
L60: Self-ligation of P93 (03.09.08: YAN)
L61: Self-ligation of P94 (03.09.08: YAN)
L62: dxs+crtE into crtBI (09.09.08: YAN)
L63: P90 (bglX) + Edinbrick 1 (09.09.08: AM)
L64: P64 (cenA) + Edinbrick 1 (09.09.08: AM)
L65: Plac+ dxs+LIMS+appY (CF)
L66: PcstA+lacZ (CF)
L68: self-ligation of P91 (sob-SU1)(CF, 22-9-8)
L69: self-ligation of P93 (sob-ISO2)(CF, 23-9-8)
L70: self-ligation of P96 (mutagenesis of cex)(CF, 23-9-8)
L73: ligation of P65 (cenA) to Edinbrick1. (CF, 24-9-8)
L74: ligation of P90 (bglX) to Edinbrick1. (CF, 24-9-8)
L75: ligation of P99 (rbs+glgC) to Edinbrick1. (CF, 25-9-8)
L76: ligation of P100 (rbs+glgC16) to Edinbrick1. (CF, 25-9-8)
L77: ligation of crtBI to dxs.crtE. (CF, 26-9-8)
L78: ligation of xylE (BBa_J33204) to PcstA. (CF, 29-9-8)
L79: ligation of Plac+lacZ' (BBa_J33207) to rbs+glgC. (CF, 29-9-8)
L80: ligation of Plac+lacZ' (BBa_J33207) to rbs+glgC16. (CF, 29-9-8)
L81: ligation of rbs to cex. (CF, 30-9-8)
L82: ligation of rbs to cenA. (CF, 30-9-8)
L83: ligation of Plac+lacZ' (BBa_J33207) to crtBI. (CF, 30-9-8)
L84: ligation of Plac+lacZ' (BBa_J33207) to dxs.crtE. (CF 30-9-8)
L87: ligation of bglX to Edinbrick2. (CF, 7-10-8)
L88: ligation of P105 (bglX with complete suffix) to Edinbrick1. (CF, 10-10-8)
L89: ligation of P105 (bglX with complete suffix) to Edinbrick2. (CF, 10-10-8)
L90: self-ligation of P107, mutagenesis of SU1. (CF, 10-10-8)
L91: self-ligation of P107, mutagenesis of ISO2. (CF, 11-10-8)
L92: repeat L83 (CF, 12-10-8)
L93: repeat L84 (CF, 12-10-8)
L94: ligation of Plac+lacZ' (BBa_J33207) to rbs+cenA (later turned out to be wrong clone). (CF, 29-9-8)
L95: ligation of Plac+lacZ' (BBa_J33207) to rbs+cex (later turned out to be wrong clone). (CF, 29-9-8)
L96: ligation of Plac+crtBI to dxs.crtE. (CF, 16-10-8)
L97: ligation of Plac+dxs.crtE to crtBI. (CF, 16-10-8)
L98: repeat of L90. (CF, 16-10-8)
L99: repeat of L91. (CF, 16-10-8)
L100: ligation of rbs to bglX. (CF, 22-10-8)
L101: ligation of P110 (rbs+cex) to Edinbrick1. (CF, 23-10-8).
L102: ligation of P111 (rbs+cenA) to Edinbrick1. (CF, 23-10-8).
L103: ligation of P112 (rbs+bglX) to Edinbrick1. (CF, 23-10-8).
L104: ligation of P113 (dxs.crtEBI) to Edinbrick1. (CF, 27-10-8).

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