Team:Utah State/Parts

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Latest revision as of 03:58, 30 October 2008

Home	The Team	The Project	Parts	Notebook	Protocols	Links

BIOBRICKS

Nine BioBricks were planned as extracts from the PHB operon. Five of these were from the PHB promoter and four were from the CAB gene cassette.

SUBMITTED BIOBRICKS

Three PHB promoter BioBricks were successfully ligated into the pSB1A3 plasmid. These BioBricks were constructed to be tested as promoters for green fluorescent protein to determine their autonomy and effectiveness. These BioBricks are labeled in the BioBrick registry as BBa_K089004, BBa_K089005, and BBa_K089006.

Miniprepped plasmid DNA from each of the BioBricks were placed into PCR tubes and labeled with their designated BioBrick number. These were placed in a 50-ml free-standing centrifuge tube and sent to iGEM for further testing and placement in the registry.

BIOBRICKS IN PROGRESS

The two remaining PHB promoter regions have been successfully amplified using PCR and are in process of ligations and transformations.

Two of the CAB cassette genes, phaA and phaB, have been successfully amplified using PCR and are in process of ligation and transformation. The phaC and phaCAB sequences are in earlier stages.

@@ Line 1: / Line 1: @@
-{|align="justify"
+{| align="center"
-|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+|align="center" |[[Image:USUheader2.gif|960 px]]
-|[[Image:Example_logo.png|200px|right|frame]]
+|}<br>
-|-
+{|style="font color="#CC3300"; background-color:#212223; cellpadding="3" cellspacing="5" border="2" bordercolor="#cd0000"border-spacing:6px; text-align:center" width="960px"
-|
+!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State|<font color="#ffffff">Home</font>]]
-''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
+!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Team|<font color="#ffffff">The Team</font>]]
-|[[Image:Team.png|right|frame|Your team picture]]
+!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Project|<font color="#ffffff">The Project</font>]]
-|-
+!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Parts|<font color="#99CCFF">Parts</font>]]
-|
+!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Notebook|<font color="#ffffff">Notebook</font>]]
-|align="center"|[[Team:Utah_State | Team Example 2]]
+!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Protocols|<font color="#ffffff">Protocols</font>]]
+!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Links|<font color="#ffffff">Links</font>]]
 |}
-<!--- The Mission, Experiments --->
+<html>
+<style type="text/css">
+body {background-image:url("https://static.igem.org/mediawiki/2008/6/63/Bkgnd14.gif");background-repeat:repeat; }
-{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
-!align="center"|[[Team:Utah_State|Home]]
-!align="center"|[[Team:Utah_State/Team|The Team]]
-!align="center"|[[Team:Utah_State/Project|The Project]]
-!align="center"|[[Team:Utah_State/Parts|Parts Submitted to the Registry]]
-!align="center"|[[Team:Utah_State/Modeling|Modeling]]
-!align="center"|[[Team:Utah_State/Notebook|Notebook]]
-|}
-(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
+</style>
+</html>
+==BIOBRICKS==
+Nine BioBricks were planned as extracts from the PHB operon.  Five of these were from the PHB promoter and four were from the CAB gene cassette.
+[[Image:PartsUSU.jpg‎|800px|center]]
+==SUBMITTED BIOBRICKS==
+Three PHB promoter BioBricks were successfully ligated into the pSB1A3 plasmid.  These BioBricks were constructed to be tested as promoters for green fluorescent protein to determine their autonomy and effectiveness. These BioBricks are labeled in the BioBrick registry as BBa_K089004, BBa_K089005, and BBa_K089006.
+[[Image:USUBiobrick1.jpg‎|320px]][[Image:USUBiobrick2.jpg‎|320px]][[Image:USUBiobrick3.jpg‎|320px]]
+Miniprepped plasmid DNA from each of the BioBricks were placed into PCR tubes and labeled with their designated BioBrick number.  These were placed in a 50-ml free-standing centrifuge tube and sent to iGEM for further testing and placement in the registry.
+[[Image:Submitted_BioBricks_2.JPG‎|320px]][[Image:Submitted_BioBricks_USU‎.JPG|320px]][[Image:Trent_Holding_BioBricks‎.JPG|320px]]
+==BIOBRICKS IN PROGRESS==
+The two remaining PHB promoter regions have been successfully amplified using PCR and are in process of ligations and transformations.
+[[Image:USUBiobrick4.jpg‎|450px]][[Image:USUBiobrick5.jpg‎|450px]]
-===Note===
+Two of the CAB cassette genes, phaA and phaB, have been successfully amplified using PCR and are in process of ligation and transformation.  The phaC and phaCAB sequences are in earlier stages.
-If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here.  This is not necessary but it may be a nice list to keep track of.
+[[Image:BiobrickPHA.jpg‎|440px]]
+[[Image:BiobrickPHAB.jpg‎|440px]]
+[[Image:BiobrickPHAC.jpg‎|430px]]
+[[Image:BiobrickPHCAB.jpg‎|430px]]