Team:Warsaw/Calendar-Main/16 September 2008

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<h3>'Hunter and prey' system tests: Competition tests</h3>
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<h4>Piotr, Emilia</h4>
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<h4>Piotr</h4>
 
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<p>Testing of <ul><li>resistance to antibiotics achieved by interacting with a protein in medium;</li><li>minimum amount of protein in medium providing resistance to ampicillin (50 ul/ml) for OmpA with A-alpha, A-omega and Z-omega;</p></li>
 
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Tests for ampicillin resistance given by protein added to the medium:
 
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Checking the minimal amount of protein added to the medium is sufficient for ampicillin (Ap 50) resistance for OmpA variants: A-alpha, A-omega and Z-omega
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Samples of liquid LB (0.25 mM IPTG; ampicillin 50 μl/ml) was inoculated with following combinations of strains:
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<ul><li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a> - A_alpha protein added ~100 μg</li>
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Rivalry Tests:  
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<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a> - Z_alpha protein added ~100 μg</li>
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Inoculation to one tube            A-omega, omega and Z-omega - A_alpha protein added ~100 μg  
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<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a> - Z_omega protein added ~100 μg</li> </ul></p>
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      (0,25IPTG; 50AP)            A-omega, omega and Z-omega - Z_alfa protein added ~100 μg  
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                                  A-alpha, alpha and Z-alpha - Z_omega protein added ~100 μg  
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'Hunter and prey' system tests: Competition tests

Piotr, Emilia

Samples of liquid LB (0.25 mM IPTG; ampicillin 50 μl/ml) was inoculated with following combinations of strains:


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