Team:Warsaw/Calendar-Main/16 September 2008

From 2008.igem.org

(Difference between revisions)
 
(7 intermediate revisions not shown)
Line 1: Line 1:
{{WarNotebook}}
{{WarNotebook}}
<!-- do not edit above me! -->
<!-- do not edit above me! -->
 +
<html>
 +
<h3>'Hunter and prey' system tests: Competition tests</h3>
 +
<h4>Piotr, Emilia</h4>
-
<h4>Piotr</h4>
 
-
<p>Testing of <ul><li>resistance to antibiotics achieved by interacting with a protein in medium;</li><li>minimum amount of protein in medium providing resistance to ampicillin (50 ul/ml) for OmpA with A-alpha, A-omega and Z-omega;</p></li>
 
-
</ul>
 
-
Tests for ampicillin resistance given by protein added to the medium:
 
-
Checking the minimal amount of protein added to the medium is sufficient for ampicillin (Ap 50) resistance for OmpA variants: A-alpha, A-omega and Z-omega
+
<p>
-
 
+
Samples of liquid LB (0.25 mM IPTG; ampicillin 50 μl/ml) was inoculated with following combinations of strains:
-
 
+
<ul><li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a> - A_alpha protein added ~100 μg</li>  
-
Rivalry Tests:
+
<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a> - Z_alpha protein added ~100 μg</li>  
-
Inoculation to one tube (0,25IPTG; 50AP)           
+
<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a> - Z_omega protein added ~100 μg</li> </ul></p>
-
                                                    <ul><li>A-omega, omega and Z-omega - A_alpha protein added ~100 μg</li>  
+
-
                                                    <li>A-omega, omega and Z-omega - Z_alfa protein added ~100 μg</li>  
+
-
                                                    <li>A-alpha, alpha and Z-alpha - Z_omega protein added ~100 μg</li> </ul>
+
   
   
-
 
+
</html>
<!-- do not remove this! -->
<!-- do not remove this! -->
{{WarNotebookEnd}}
{{WarNotebookEnd}}

Latest revision as of 21:15, 26 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


'Hunter and prey' system tests: Competition tests

Piotr, Emilia

Samples of liquid LB (0.25 mM IPTG; ampicillin 50 μl/ml) was inoculated with following combinations of strains:


Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/16_September_2008"