Team:Warsaw/Calendar-Main/2 September 2008
From 2008.igem.org
(Difference between revisions)
(11 intermediate revisions not shown) | |||
Line 3: | Line 3: | ||
<html> | <html> | ||
- | <h3>Purification of proteins: | + | <h3>Purification of proteins: A-alpha</h3><h4>Piotr, Emilia</h4> |
- | Piotr </ | + | <p><ol><li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A_alpha</a> in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> strain from overnight culture inoculated in fresh LB and cultured until OD=0.5.</li> |
- | <p><ol><li> | + | <li>Culture was induced with different concentrations of IPTG at 22°C and 37°C.</li> |
- | <li> | + | <li>Samples were collected twice: after 3h and next day (samples were centrifuged and frozen).</li></ol></p> |
- | <li>Samples were collected twice: after 3h and next day (samples were centrifuged and frozen)</li></ol></p> | + | |
Latest revision as of 21:02, 26 October 2008
Purification of proteins: A-alphaPiotr, Emilia
|