Team:Warsaw/Calendar-Main/12 May 2008

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<html><h3>Preparation of pMPMT5+AID construct</h3>
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<html><h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPMT5+AID</a> construct</h3>
<h4>Michał K.</h4>
<h4>Michał K.</h4>
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<li> <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#electrotransform">electroporation</a> with ligation (pMPM-T5+AID).</li>
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<li> <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#electrotransform">electroporation</a> with ligation (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPMT5+AID</a>).</li>
<li>Plating on LB + tetracycline.</li></ol></p>
<li>Plating on LB + tetracycline.</li></ol></p>

Latest revision as of 16:11, 12 October 2008

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Preparation of pMPMT5+AID construct

Michał K.

  1. E. coli TOP10 electroporation with ligation (pMPMT5+AID).
  2. Plating on LB + tetracycline.

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