Team:Warsaw/Calendar-Main/12 June 2008

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<h3> Blue/white and rifampicin test </h3>
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<h3>Blue/white and rifampicin test #1</h3>
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<h4>Michał L., Ewa, Marcin</h4>
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<h4>Michał L., Ewa, Piotr</h4>
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<ol>
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<p>Blue colonies of each transformant strain were counted.<br>
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<li>Blue colonies of each transformant strain were counted.<br>
Results (% of blue colonies):<br>
Results (% of blue colonies):<br>
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pMPMT5 100%<br>
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<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPMT5-omega</a> 99%<br>
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pMPMT5-AID 98%<br>
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<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPMT5-AID</a> 98%<br>
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pMPMT5-AIDT7 76%<br>
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<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5-AIDT7</a> 76%<br>
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pMPMT5-AID+T7 0.2%<br>
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<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a> 0.2%<br>
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</li>
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</p>
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<li>Plating transformant's cultures from the previous day on rifampicin.</li>
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<li>OD measurement.</li></ol></p>
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</p>
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<table id="result">
 
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<thead><b> Results of rifampicin test</b><br></thead>
 
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<tr><th>Strain</th><th>Inducer / repressor</th><th>Number of colonies on rifampicin</th></tr>
 
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<tr><th>Top10</th><td>none</td><td>3</td></tr>
 
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<tr><th>Top10 pMPM-AID</th><td>0.1% Arabinose</td><td>63</td></tr>
 
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<tr><th>Top10 pMPM-AID</th><td>none</td><td>9</td></tr>
 
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<tr><th>Top10 pMPM-A+T13</th><td>0.1% Arabinose</td><td>127</td></tr>
 
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<tr><th>Top10 pMPM-A+T13</th><td>none</td><td>7</td></tr>
 
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<tr><th>Top10 pMPM-AT6</th><td>0.1% Arabinose</td><td>25</td></tr>
 
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<tr><th>Top10 pMPM-AT6</th><td>none</td><td>14</td></tr>
 
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</table>
 

Latest revision as of 14:49, 26 October 2008

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Blue/white and rifampicin test #1

Michał L., Ewa, Piotr

  1. Blue colonies of each transformant strain were counted.
    Results (% of blue colonies):
    pMPMT5-omega 99%
    pMPMT5-AID 98%
    pMPMT5-AIDT7 76%
    pMPMT5-AID+T7 0.2%
  2. Plating transformant's cultures from the previous day on rifampicin.
  3. OD measurement.

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