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Team:Warsaw/Calendar-Main/28 May 2008
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| - | <html><h3>Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7</h3> | + | <html> |
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| + | <h3>Rifampicin test #3</h3> | ||
| + | <h4>Michał L., Ewa, Marcin</h4> | ||
| + | <p><ol> | ||
| + | <li> Inoculation of liquid LB with E.coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPM-T5+AID</a> </li> | ||
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| + | <li> Inoculation of liquid LB with E.coli <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying | ||
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| + | <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A+AID</a></li></ol></p><br> | ||
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| + | <h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPMT5+AID-T7</a></h3> | ||
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
<p><ol> | <p><ol> | ||
| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5 with transcriptional fusion.</li> | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPM-T5 with transcriptional fusion</a>.</li> |
| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5 with translational fusion.</li> | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPM-T5 with translational fusion</a>.</li> |
| - | <li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with transcriptional fusion with EcoRI (EcoRI buffer).</li> | + | <li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPM-T5 with transcriptional fusion</a> with EcoRI (EcoRI buffer).</li> |
| - | <li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translational fusion with BamHI (BamHI buffer).</li> | + | <li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAIDT7>pMPM-T5 with translational fusion</a> with BamHI (BamHI buffer).</li> |
<li> Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/28_May_2008#fig1">Fig. 1</a> and <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/28_May_2008#fig2">Fig. 2</a>). </li> | <li> Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/28_May_2008#fig1">Fig. 1</a> and <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/28_May_2008#fig2">Fig. 2</a>). </li> | ||
<li> Choice of proper clones and preparation for sequencing.</li> | <li> Choice of proper clones and preparation for sequencing.</li> | ||
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| - | <center><a name="fig1"><img src="https://static.igem.org/mediawiki/2008/6/62/Trawienie_06_05_2008.jpg"/></a></center> | + | <center><a name="fig1"><img src="https://static.igem.org/mediawiki/2008/6/62/Trawienie_06_05_2008.jpg" width=190/></a></center> |
<var><b>Fig. 1.</b> Here we found the clone with translational fusion on pMPM. These are different isolations in each lane. We have been working with the AT6 ever since. <br> | <var><b>Fig. 1.</b> Here we found the clone with translational fusion on pMPM. These are different isolations in each lane. We have been working with the AT6 ever since. <br> | ||
Digest was conducted with BamHI</var> | Digest was conducted with BamHI</var> | ||
<div align=center> | <div align=center> | ||
| - | <a name="fig2"><img src="https://static.igem.org/mediawiki/2008/d/d6/Translation_fusion_digest_Eco.jpg"width= | + | <a name="fig2"><img src="https://static.igem.org/mediawiki/2008/d/d6/Translation_fusion_digest_Eco.jpg"width=250/></a></div> |
<var><b>Fig. 2.</b> 2 to 6 - Control digests of few clones with transcriptional fusion; <br>7 - pMPMT5-AID; all plasmids digested with EcoRI. </var> | <var><b>Fig. 2.</b> 2 to 6 - Control digests of few clones with transcriptional fusion; <br>7 - pMPMT5-AID; all plasmids digested with EcoRI. </var> | ||
Latest revision as of 16:41, 28 October 2008
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Rifampicin test #3Michał L., Ewa, Marcin
Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7Michał K.
 Digest was conducted with BamHI  7 - pMPMT5-AID; all plasmids digested with EcoRI.
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