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Wisconsin: Lignin Project/16 July 2008
From 2008.igem.org
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|'''Team Sorbitol'''<br> | |'''Team Sorbitol'''<br> | ||
Growth curves directly comparing Wt+(MG1655) and tpiA knockout in C-media.<br> | Growth curves directly comparing Wt+(MG1655) and tpiA knockout in C-media.<br> | ||
| - | + | Start PCR srl operon from genomic DNA provided by Dr. Pfleger instead of MG1655. Still no band is shown on the gel.<br> | |
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| + | '''Team Fungus'''<br> | ||
Ran gradient PCR reaction using TAQ and "Good Stuff" template dilute (1:10).<br> | Ran gradient PCR reaction using TAQ and "Good Stuff" template dilute (1:10).<br> | ||
| + | Started overnight cultures from transformation.<br> | ||
| + | Ran touchdown PCR using Taq using "good stuff" as template (correct band from 7/11).<br> | ||
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Latest revision as of 03:39, 29 October 2008
| Team Sorbitol Growth curves directly comparing Wt+(MG1655) and tpiA knockout in C-media.
|
