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Wisconsin: Lignin Project/25 July 2008
From 2008.igem.org
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Extracted the digestion and performed a ligation which ran overnight at 4C<br> | Extracted the digestion and performed a ligation which ran overnight at 4C<br> | ||
Another attempt to PCR srl Operon success! 7 of 8 reactions correct band placement on gel<br> | Another attempt to PCR srl Operon success! 7 of 8 reactions correct band placement on gel<br> | ||
| + | Digestion and Ligations performed. Success! <br> | ||
'''Team Fungus:'''<br> | '''Team Fungus:'''<br> | ||
Made 1 : 10 dilutions of C and N-limited cDNA in 10mM Tris-CL (pH 8.0) to preserve the little we have left.<br> | Made 1 : 10 dilutions of C and N-limited cDNA in 10mM Tris-CL (pH 8.0) to preserve the little we have left.<br> | ||
| - | + | Ran gradient PCR with Taq with diluted cDNA.<br> | |
| + | Ran nested PCR with Taq with diluted cDNA.<br> | ||
| + | Ran nested touchdown PCR with Taq with diluted cDNA.<br> | ||
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Latest revision as of 09:26, 29 October 2008
| Team Sorbitol: Digested the srlD and pET28a with BamHI and HindIII |
