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Wisconsin: Lignin Project/31 July 2008
From 2008.igem.org
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Competent cells for MG1655, pBAD30,pBAD18, and DH5a<br> | Competent cells for MG1655, pBAD30,pBAD18, and DH5a<br> | ||
<br> | <br> | ||
| - | + | '''Team Fungus:'''<br> | |
Ran digestion of pET28a and ran out on .75% agrose gel. All bands were correct size. <br> | Ran digestion of pET28a and ran out on .75% agrose gel. All bands were correct size. <br> | ||
Used the QIAquick gel extraction kit to purify the double digested vector band. Spec reading of 14 ng/uL.<br> | Used the QIAquick gel extraction kit to purify the double digested vector band. Spec reading of 14 ng/uL.<br> | ||
Ran overnight ligation at 4 degrees C. | Ran overnight ligation at 4 degrees C. | ||
|} | |} | ||
Latest revision as of 03:11, 29 October 2008
| Team Sorbitol: Competent cells for MG1655, pBAD30,pBAD18, and DH5a |
