Wisconsin:Lignin Project/28 August 2008

From 2008.igem.org

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New primers arrived for gap between srlA, and srlE.<br>
New primers arrived for gap between srlA, and srlE.<br>
Performed new sequencing PCR reaction.<br>
Performed new sequencing PCR reaction.<br>
 +
srlD PCR product was digested with biobrick vector pSB1AK3 recieved from the registry.<br>
 +
Digestion was verified on a gel and purified through gel extraction. <br>
 +
Overnight ligation of insert into pSB1AK3 was allowed to run overnight.<br>
 +
 +
'''Team Fungus:'''<br>
 +
Ran Azure B assay on 96 well plate.<br>
 +
Ran SDS PAGE gel.<br>
 +
Ran western blot with T7 tag antibody.<br>
 +
Grew overnight cultures for 5.r1.23 and 5.r1.20
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Latest revision as of 09:50, 29 October 2008

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Team Sorbitol:

New primers arrived for gap between srlA, and srlE.
Performed new sequencing PCR reaction.
srlD PCR product was digested with biobrick vector pSB1AK3 recieved from the registry.
Digestion was verified on a gel and purified through gel extraction.
Overnight ligation of insert into pSB1AK3 was allowed to run overnight.

Team Fungus:
Ran Azure B assay on 96 well plate.
Ran SDS PAGE gel.
Ran western blot with T7 tag antibody.
Grew overnight cultures for 5.r1.23 and 5.r1.20

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