Team:Warsaw/Calendar-Main/16 June 2008
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- | <h3>Preparation of constructs | + | <h3>Preparation of constructs: OmpA_alpha and OmpA_omega</h3> |
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
<p> | <p> | ||
<ol> | <ol> | ||
- | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on pB30D plasmid with | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pB30D>pB30D</a> plasmid with |
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a> | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a> | ||
primers (15 cycles, elongation duration 45 s, annealing temperature 63°C). </li> | primers (15 cycles, elongation duration 45 s, annealing temperature 63°C). </li> | ||
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<li>Electrophoresis to estimate the concentration of isolated DNA.</li> | <li>Electrophoresis to estimate the concentration of isolated DNA.</li> | ||
</ol></p> | </ol></p> | ||
- | <h3> Blue/white test </h3> | + | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/a/a3/PCR_Ompa_Alpha_Omega_WAW.jpg" width=300/></a> |
+ | <var><b>Fig. 1.</b> PCR products: linker_alpha (lane 2), linker_omega (lane 3), OmpA_linker (lane 4)</var> | ||
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+ | <h3> Blue/white and rifampicin test #1</h3> | ||
<h4>Michał L., Ewa</h4> | <h4>Michał L., Ewa</h4> | ||
<ol> | <ol> | ||
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<a name="fig2"><img src="https://static.igem.org/mediawiki/2008/0/0c/Colony_PCR_pZC_WAW.jpg" width=400/></a> | <a name="fig2"><img src="https://static.igem.org/mediawiki/2008/0/0c/Colony_PCR_pZC_WAW.jpg" width=400/></a> |
Latest revision as of 14:50, 26 October 2008
Preparation of constructs: OmpA_alpha and OmpA_omegaMichał K.
Blue/white and rifampicin test #1Michał L., Ewa
Fig. 2. 1 - DNA ladder; 2 to 11 - colony PCR products (lacZ' gene) from blue and white colonies.
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