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Team:Warsaw/Calendar-Main/24 June 2008
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| - | <h3>Preparation of constructs | + | <h3>Preparation of constructs: OmpA_alpha and OmpA_omega</h3> |
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
<ol><li> PCR on isolated plasmids with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a> | <ol><li> PCR on isolated plasmids with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a> | ||
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| - | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/7/78/Omp_colony_PCR_WAW.jpg" width= | + | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/7/78/Omp_colony_PCR_WAW.jpg" width=350/></a> |
<var><b>Fig. 1.</b> PCR product - searching for fusions with OmpA protein. Lower lanes - OmpA_alpha, upper - OmpA_omega.</var> | <var><b>Fig. 1.</b> PCR product - searching for fusions with OmpA protein. Lower lanes - OmpA_alpha, upper - OmpA_omega.</var> | ||
Latest revision as of 16:53, 28 October 2008
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Preparation of alpha-A and omega-A fusionsMichał L., Ewa, MarcinStill no success. We need to run gradient PCR to find optimal reaction conditions. Preparation of constructs: OmpA_alpha and OmpA_omegaMichał K.
Fig. 1. PCR product - searching for fusions with OmpA protein. Lower lanes - OmpA_alpha, upper - OmpA_omega.
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