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Team:Warsaw/Calendar-Main/27 August 2008
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| - | <br> | + | <h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4> |
| + | Results of growth from previous day | ||
| + | <br><br> | ||
| + | <table id="result"> | ||
| + | <tr><th>OmpA variant ("hunter")</th><th>"Prey" variant</th><th>Growth with prey</th><th>Growth without prey</th></tr> | ||
| + | <tr><th>OmpA-alpha</th><td>His+Z+omega</td><td>+</td><td>-</td></tr> | ||
| + | <tr><th>OmpA-alpha</th><td>His+Z+alfa</td><td>-</td><td>-</td></tr> | ||
| + | <tr><th>OmpA-A-alpha</th><td>His+Z+omega</td><td>+</td><td>-</td></tr> | ||
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| + | </table> | ||
<h3>Cloning of protein A DNA to <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA-alpha</a> plasmid</h3><h4>Michał K.</h4> | <h3>Cloning of protein A DNA to <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA-alpha</a> plasmid</h3><h4>Michał K.</h4> | ||
| - | <ol><li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pGeneart-A>pGeneart+A</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+ | + | <ol><li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pGeneart-A>pGeneart+A</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA-alpha</a> with NdeI and NotI (Orange buffer), pET15b was also <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing"> dephosphorylated</a>. </li> |
| - | <li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands (400 bp for A lane and 6200 bp for pET15b lane). </li> | + | <li> Gel electrophoresis of digested plasmids (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/27_August_2008#fig1">Fig. 1.</a>) and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands (400 bp for A lane and 6200 bp for pET15b lane). </li> |
| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b-Alpha and A</a>. </li> |
<li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>. </li> | <li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>. </li> | ||
<li> Transformants plating on LB + ampicillin.</li> | <li> Transformants plating on LB + ampicillin.</li> | ||
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| + | <a name="fig1"><img src=" | ||
| + | https://static.igem.org/mediawiki/2008/1/1b/Go29.jpg" width=300/></a> <var><b>Fig. 1.</b> NdeI/NotI digest of plasmid pGeneart+A<br> | ||
| + | 1. Marker<br> | ||
| + | 2. Digested plasmid pGeneart+A<br></var> | ||
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</html> | </html> | ||
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Tests for ampicillin resistance given by protein added to mediumPiotrResults of growth from previous day
Cloning of protein A DNA to pET15b+OmpA-alpha plasmidMichał K.
 Fig. 1. NdeI/NotI digest of plasmid pGeneart+A1. Marker 2. Digested plasmid pGeneart+A
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