Waterloo/9 September 2008

From 2008.igem.org

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(New page: <u>080908 Mon</u><br> [x] check Sunday's transformation results<br> Putative good constructs yet to be sequenced:<br> [x] inoculate culture from transformants of parts for sequencing (an...)
 
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<u>080908 Mon</u><br>
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<u>080909 Tue</u> <br>
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[x] check Sunday's transformation results<br>
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[x] check crossover PCR primers<br>
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Putative good constructs yet to be sequenced:<br>
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Parts being transferred to a BioBrick vector:<br>
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[x] inoculate culture from transformants of parts for sequencing (and making glycerol stock)<br>
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[x] colony PCR on transformants of ligation mixes<br>
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[x] inoculate culture from transformants of synthesized parts in original GeneArt vector (for glycerol stock)<br>
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[x] run products on a gel<br>
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[x] save glycerol stock of Plac-rfp"<br>
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[x] kit prep of parts for sequencing<br>
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[x] make glycerol stocks<br>
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[x] miniprep pSB2K3(gene6) and pSB2K3(PmeI) for diag digest and gel<br>
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[x] send above pDNA for sequencing<br>
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[x] digest of above miniprep<br>
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[x] gel of above digest<br>
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[x] for each part, subculture one correct-looking sample in duplicate to kit prep and save stock of tomorrow<br>
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[] design crossover PCR primers<br>
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[x] reorganize freezer boxes (did enzyme/PCR boxes)<br>
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- Half done<br>
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[x] pick time for lab meeting<br>
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- Meeting has been set for Wednesday Sept. 10th at 5:30<br>
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Latest revision as of 19:24, 20 October 2008

080909 Tue

[x] check crossover PCR primers

Parts being transferred to a BioBrick vector:
[x] colony PCR on transformants of ligation mixes
[x] run products on a gel

[x] kit prep of parts for sequencing
[x] make glycerol stocks

[x] send above pDNA for sequencing

[x] reorganize freezer boxes (did enzyme/PCR boxes)

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