Team:Mississippi State

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| style="padding:0 .3em; border:solid 1px #a3bfb1; border-bottom: 0; text-align:center; font-weight:bold; background-color:#f5fffa;" width="23.5%" | '''MSUSB iGEM 08'''<br />
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| style="border-bottom:1px solid #a3bfb1; background:none;" width="2%" | &nbsp;
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[[Team:Mississippi State/Team|Team]]
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| style="border-bottom:1px solid #a3bfb1; background:none;" width="2%" | &nbsp;
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| style="padding:0 .3em; background-color:#cedff2; font-size:90%; border:solid 1px #a3bfb1; border-bottom:1px solid #a3bfb1; text-align:center" width="23.5%" | [[Team:Mississippi State/Project|Project]]
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| style="border-bottom:1px solid #a3bfb1; background:none;" width="2%" | &nbsp;
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| style="padding:0.3em; font-size:90%; background-color:#cedff2; border:solid 1px #a3bfb1; border-bottom:1px solid #a3bfb1; text-align:center" width="23.5%" | [[Team:Mississippi State/Papers|Papers and Notebook]]
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'''Lignin is a ubiquitous, extremely complex biopolymer found in plant cells.  It is the most recalcitrant part of the cell wall, and only a few organisms possess the machinery necessary for its degradation.  As a result, a huge proportion of the earth's biomass resources are trapped in a highly degradation resistant lignin matrix.  To make these resources viable for energy and chemical needs, lignin must be broken down to separate the chemical components of biomass.'''
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=Mississippi State University Synthetic Biology=
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[[Image:Argaiv.JPG|350 px|right]]
 
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'''Lignin Project'''
 
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*[[Team:Mississippi_State/What the Alert Box Says|What the Alert Box Says]]
 
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*[http://en.wikipedia.org/wiki/Wikipedia:How_to_edit_a_page Wiki How-To]
 
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*[[Team:Mississippi_State/On Pichia Pastoris|On Pichia Pastoris]]
 
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*[[Team:Mississippi_State/Making BioBricks|Making BioBricks]]
 
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*[[Team:Mississippi_State/Timeline of Things To Do|Timeline of Things To Do]]
 
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*[http://usasearch.gov/search?affiliate=fpl.fs.fed.us&v%3Aproject=firstgov&query=lignin+peroxidase| Good resource for Methods]
 
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*[[Team:Mississippi_State/General things needed for Lab|General things needed for Lab]]
 
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*[http://scielo.isciii.es/scielo.php?pid=S1139-67092005000300007&script=sci_arttext&tlng=e Must read article]
 
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*[http://www.jbc.org/cgi/reprint/274/15/10324 ...and another]
 
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*[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1302970 Dynamics of LiP]
 
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*[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TG1-45HVTH7-5&_user=1512607&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000053407&_version=1&_urlVersion=0&_userid=1512607&md5=01a96f4cfb9afa50b7bde2bd7295a4c7 Dynamics of MnP]
 
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*[http://www.jbc.org/cgi/content/full/278/41/39726 Mechanics of LiP]
 
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*[http://pubs.acs.org/cgi-bin/abstract.cgi/chreay/2001/101/i11/abs/cr000115l.html and another]
 
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*Google "how long does Lignin Peroxidase last"!!!
 
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Mississippi State seeks to benefit the uses of non-food material biomass as a source of energy. Though ethanol and other biofuels offer an alternative to fossil fuels, their extraction from food crops is unrealistic and puts enormous economic strain on both food products and the further development of natural fuels. Biomass waste contains a huge amount of unused cellulose and hemicellulose, the raw materials for biofuel production. In addition, Lignin has been shown to be a source of biogasoline, which conforms better than any current biofuel to the existing energy infrastructure. As a result, this project will develop a better method for natural degradation of biomass to reduce the costs and complications involved with current methods.
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We have isolated a single gene from the Lignin Peroxidase gene family.  This gene has been sequenced and cloned into Pichia Pastoris for testing.  This gene produces the Lignin Peroxidase enzyme responsible for initiating the breakdown of lignin.  Upon this fundamental research can be built a characterized and controllable system for the breakdown of biomass.  Our project is vital to developing a biological process for degrading biomass.  Lignin Peroxidase initiates this process, so we see fit that this technology must be the first developed.  We want to make our resources a reality, and this project is the first step.
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{{ #calendar: title=Team:Mississippi_State |year=2008 | month=06 }}
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==Sites of Interest==
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[[Team:Mississippi_State | MSU iGEM 08 Team Page]]
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[https://2008.igem.org/Main_Page iGEM 08]
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[http://partsregistry.org/Main_Page The Registry]
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Msusb_logo.JPG|300 px|right]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|align="center"|[[Team:Mississippi_State | Team Example 2]]
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Mississippi_State|Home]]
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!align="center"|[[Team:Mississippi_State/Team|The Team]]
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!align="center"|[[Team:Mississippi_State/Project|The Project]]
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!align="center"|[[Team:Mississippi_State/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Mississippi_State/Modeling|Modeling]]
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!align="center"|[[Team:Mississippi_State/Notebook|Notebook]]
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(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
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Latest revision as of 18:19, 13 October 2008

Msusbbubble.JPG
MSUSB iGEM 08
 

Team

  Project   Papers and Notebook



Lignin is a ubiquitous, extremely complex biopolymer found in plant cells. It is the most recalcitrant part of the cell wall, and only a few organisms possess the machinery necessary for its degradation. As a result, a huge proportion of the earth's biomass resources are trapped in a highly degradation resistant lignin matrix. To make these resources viable for energy and chemical needs, lignin must be broken down to separate the chemical components of biomass.


Before.JPG
RethinkBiofuels.JPG


Mississippi State seeks to benefit the uses of non-food material biomass as a source of energy. Though ethanol and other biofuels offer an alternative to fossil fuels, their extraction from food crops is unrealistic and puts enormous economic strain on both food products and the further development of natural fuels. Biomass waste contains a huge amount of unused cellulose and hemicellulose, the raw materials for biofuel production. In addition, Lignin has been shown to be a source of biogasoline, which conforms better than any current biofuel to the existing energy infrastructure. As a result, this project will develop a better method for natural degradation of biomass to reduce the costs and complications involved with current methods.


We have isolated a single gene from the Lignin Peroxidase gene family. This gene has been sequenced and cloned into Pichia Pastoris for testing. This gene produces the Lignin Peroxidase enzyme responsible for initiating the breakdown of lignin. Upon this fundamental research can be built a characterized and controllable system for the breakdown of biomass. Our project is vital to developing a biological process for degrading biomass. Lignin Peroxidase initiates this process, so we see fit that this technology must be the first developed. We want to make our resources a reality, and this project is the first step.


Msuigem08.JPG