Team:Caltech/Protocols/FimE

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# Grow 5mL LB cultures with appropriate antibiotics overnight.
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Insert protocol here
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# Backdilute culture 1:100 into fresh 5mL LB with appropriate antibiotics.
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# When the cultures reach 0.4 OD at 600nm, add 0.1% arabinose and grow at 37C for 1 hour.
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# Wash and resuspend cells in phosphate buffer solution (100mM, 7.0pH).
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# Measure GFP fluorescence of 200 uL of culture at 498nm on plate reader.
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FimE Inversion Assay


  1. Grow 5mL LB cultures with appropriate antibiotics overnight.
  2. Backdilute culture 1:100 into fresh 5mL LB with appropriate antibiotics.
  3. When the cultures reach 0.4 OD at 600nm, add 0.1% arabinose and grow at 37C for 1 hour.
  4. Wash and resuspend cells in phosphate buffer solution (100mM, 7.0pH).
  5. Measure GFP fluorescence of 200 uL of culture at 498nm on plate reader.
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