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Alberta NINT/13 June 2008
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| + | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
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| + | [[Alberta_NINT/12_June_2008 | < Previous entry]] | [[Alberta_NINT/14_June_2008 | Next entry >]] | ||
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lab work (SD): | lab work (SD): | ||
Using the transformation completed by Wayne (12/06/07) of XL1-B cells with I732018 and the overnight | Using the transformation completed by Wayne (12/06/07) of XL1-B cells with I732018 and the overnight | ||
cultures, DNA was isolated by QIAprep standard miniprep protocol. | cultures, DNA was isolated by QIAprep standard miniprep protocol. | ||
I732018 was sequenced. | I732018 was sequenced. | ||
| - | I732018 was digested with XbaI + PstI. | + | I732018 was digested with XbaI + PstI. No evidence of DNA in the gel. Retry! |
| + | [[Image:NINT_SD_p37.jpg|750 px]] | ||
lab work (JD): | lab work (JD): | ||
Quantitated K102005/BamH1 and Nco1 | Quantitated K102005/BamH1 and Nco1 | ||
| - | Ligated K102005/BamH1 and Nco1 with TA0/BamH1 and Nco1 (1/100 dilution) | + | Ligated K102005/BamH1 and Nco1 with TA0/BamH1 and Nco1 (1/100 dilution) to create plasmid K102007 |
| - | Transformed XL1-B cells with | + | Transformed XL1-B cells with K102007 and negative control |
Plated cells and left them for O/N | Plated cells and left them for O/N | ||
Latest revision as of 22:49, 11 October 2008
< Previous entry | Next entry >
lab work (SD):
Using the transformation completed by Wayne (12/06/07) of XL1-B cells with I732018 and the overnight
cultures, DNA was isolated by QIAprep standard miniprep protocol.
I732018 was sequenced.
I732018 was digested with XbaI + PstI. No evidence of DNA in the gel. Retry!
lab work (JD):
Quantitated K102005/BamH1 and Nco1
Ligated K102005/BamH1 and Nco1 with TA0/BamH1 and Nco1 (1/100 dilution) to create plasmid K102007
Transformed XL1-B cells with K102007 and negative control
Plated cells and left them for O/N
