Team:UCSF

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Untitled Document

CHROMATIN MEMORIES

A new tool for Synthetic Biology

Epigenetic control of gene expression

The cells of higher eukaryotes utilize chromatin state to encode "permanent" epigenetic changes in gene expression. For example, signals received by a cell during the course of development can induce the partitioning of the genome into accessible (euchromatin) and inaccessible (heterochromatin) regions that specify the fate of that cell. This epigenetic profile, in which blocks of gene are "silenced" by heterochromatin, is stably maintained and inherited by daughter cells. Thus, chromatin state provides a higher level of gene expression control that is regional (acting on many genes at once), dominant over transcription factors, ultra-cooperative (all or none), and highly stable (memory). Engineerable control over chromatin state would clearly be a powerful tool for Synthetic Biology.

We have constructed and characterized a synthetic silencing system in S. cerevisiae in which we can inducibly silence specific loci in the genome.

This foundational technology will facilitate the construction of complex genetic circuits with memory, and has potential application in the engineering of cell differentiation in higher eukaryotes.

More...

OUR PROJECT

Synthetic Chromatin Bit

Design

Properties

Modeling

Strengthening Silencing

Higher-Order Systems

Materials and Methods

FAQs about our Project

Human Practices

OUR TEAM

Team Members

Summer Experience

Notebooks

Aar1 Cloning System

Parts submitted to the Registry

FAQs about our Team

UCSF iGEM 2008 is sponsored by...

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-<p align="center" style="font-size:24pt"> CHROMATIN MEMORIES</p>
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+<p align="center"><img src="https://static.igem.org/mediawiki/2008/0/0e/UCSF2008logo2.jpg" width="276" height="288" align="middle" /></p>
+<p  align="center" class="style2">CHROMATIN MEMORIES<br></br>A new tool for Synthetic Biology</p>
+<blockquote>
+  <br>
+  <br>
+  <br>
+      <h2 align="left">Epigenetic control of gene expression</h2>
+      <p align="left">The cells of higher eukaryotes utilize chromatin state to encode &quot;permanent&quot; epigenetic changes in gene expression.  For example, signals received by a cell during the course of development can induce the partitioning of the genome into accessible (euchromatin) and inaccessible (heterochromatin) regions that specify the fate of that cell.  This epigenetic profile, in which blocks of gene are &quot;silenced&quot; by heterochromatin, is stably maintained and inherited by daughter cells.  Thus, chromatin state provides a higher level of gene expression control that is regional (acting on many genes at once), dominant over transcription factors, ultra-cooperative (all or none), and highly stable (memory).  Engineerable control over chromatin state would clearly be a powerful tool for Synthetic Biology.  </p>
+      <p align="left"><strong>We have constructed and characterized a synthetic silencing system in <em>S. cerevisiae</em> in which we can inducibly silence specific loci in the genome. </strong></p>
+      <p align="left">This foundational technology will facilitate the construction of complex genetic circuits with memory, and has potential application in the engineering of cell differentiation in higher eukaryotes.</p>
+    <p align="right"><a href="https://2008.igem.org/Team:UCSF/Project">More...</a></p>
+      <p align="right">&nbsp;</p>
+      <table width="870" border="0" cellpadding="3">
+        <tr>
+          <td width="202" height="208"><img src="https://static.igem.org/mediawiki/2008/b/b6/Our_project.jpg" alt="" width="200" height="201" /></td>
+          <td width="650"><h3>&nbsp;</h3>
+            <blockquote>
+              <h3>OUR PROJECT</h3>
+              <blockquote>
+                <h4><a href="https://2008.igem.org/Team:UCSF/Project">Synthetic Chromatin Bit</a></h4>
+                <ul>
+                  <ul>
+                    <li><a href="https://2008.igem.org/Team:UCSF/Synthetic Chromatin Design">Design</a></li>
+                    <li><a href="https://2008.igem.org/Team:UCSF/Synthetic Chromatin Properties">Properties</a></li>
+                    <li><a href="https://2008.igem.org/Team:UCSF/Modeling">Modeling</a></li>
+                    <li><a href="https://2008.igem.org/Team:UCSF/Strengthening Silencing">Strengthening Silencing</a></li>
+                    <li><a href="https://2008.igem.org/Team:UCSF/Higher_order_systems">Higher-Order Systems</a></li>
+                    <li><a href="https://2008.igem.org/Team:UCSF/Materials and Methods">Materials and Methods</a></li>
+                  </ul>
+                </ul>
+                <h4><a href="https://2008.igem.org/FAQs_about_our_Project">FAQs about our Project</a></h4>
+                <h4><a href="https://2008.igem.org/Team:UCSF/Human Practices">Human Practices</a></h4>
+              </blockquote>
+            </blockquote>
+          <p>&nbsp;</p></td>
+        </tr>
+      </table>
+      <table width="870" border="0" cellpadding="3">
+        <tr>
+          <td width="202" height="208"><img src="https://static.igem.org/mediawiki/2008/f/f5/Our_team.jpg" alt="" width="200" height="200" /></td>
+          <td width="650"><h3>&nbsp;</h3>
+              <blockquote>
+                <h3>OUR TEAM</h3>
+                <blockquote>
+                  <h4><a href="https://2008.igem.org/Team:UCSF/Team">Team Members</a></h4>
+                  <h4><a href="https://2008.igem.org/Team:UCSF/Summer Experience">Summer Experience</a></h4>
+                  <h4><a href="https://2008.igem.org/Team:UCSF/Notebook">Notebooks</a></h4>
+                  <h4><a href="https://2008.igem.org/Everything_you_ever_wanted_to_know_about_AarI">Aar1 Cloning System</a></h4>
+                  <h4><a href="https://2008.igem.org/Team:UCSF/Parts">Parts submitted to the Registry</a></h4>
+                  <h4><a href="https://2008.igem.org/FAQs_about_our_Team">FAQs about our Team</a></h4>
+                </blockquote>
+              </blockquote>
+          <p>&nbsp;</p></td>
+        </tr>
+      </table>
+<br><br></br></br>
+  </blockquote>
+</body>
+</html>
-<div style="font-size:12pt; position:relative; left:40px"> Our project focused on blah blah blah blah blah</div>
-<br><br><br><br><br><br><br><br><br><br><br><div><hr align="center"; style="height:2px" "color:#333333">
-====OUR PROJECT====
-=====[[Team:UCSF/Project|Project Description]]=====
-=====[[Synthetic Chromatin Bit]]=====
-:*[[UCSF System Design|Design]]
-:*[[UCSF System Properties| Properties]]
-:*[[UCSF Higher-Order Systems|Higher-Order Systems]]
-:*[[FAQs about our Project|FAQs]]
-=====[[Human Practices]]=====
+'''UCSF iGEM 2008 is sponsored by...'''
-====OUR TEAM====
-:*[[Team:UCSF/Team|Team Members]]
-:*[[Team:UCSF/Team|Individual Contributions]]
-:*[[UCSF Notebooks|Notebooks]]
-:*[[UCSF Resources|Resources]]
-:*[[FAQs about our Team|FAQs]]
+<html>
-<!--- The Mission, Experiments --->
+<p align="center"><img src="https://static.igem.org/mediawiki/2008/1/16/Sponsors.png" width="650" height="129" align="middle" /></p></html>
-{| style="color:#333333;background-color:#cccccc;" cellpadding="3" cellspacing="3" border="0" bordercolor="#231f26" width="99%" align="center"
-!align="center"|[[Team:UCSF|Home]]
-!align="center"|[[Team:UCSF/Team|The Team]]
-!align="center"|[[Team:UCSF/Project|The Project]]
-!align="center"|[[Team:UCSF/Parts|Parts Submitted to the Registry]]
-!align="center"|[[Team:UCSF/Modeling|Modeling]]
-!align="center"|[[Team:UCSF/Human Practices|Human Practices]]
-!align="center"|[[Team:UCSF/Notebook|Notebooks]]
-|}