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Team:Chiba/Calendar-Home/27 August 2008
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(New page: >Home | Notebook 26 August 2008 <|> 28 August 2008 ==Labora...) |
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===Team:Communication=== | ===Team:Communication=== | ||
[[Team:Chiba/protocol/digestion|Digestion Test]] | [[Team:Chiba/protocol/digestion|Digestion Test]] | ||
| - | ::Sample DNA : | + | ::Sample DNA :[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] |
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
| Line 21: | Line 21: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>PstI</td> |
<td>-</td><td>-</td><td>0.5</td> | <td>-</td><td>-</td><td>0.5</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>EcoRI</td> |
<td>-</td><td>0.5</td><td>0.5</td> | <td>-</td><td>0.5</td><td>0.5</td> | ||
</tr> | </tr> | ||
| Line 42: | Line 42: | ||
<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>1μl</td><td>10μl</td><td>10μl</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 65: | Line 65: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>H<sub>2</sub>O</td> |
<td>4</td><td>-</td><td>-</td> | <td>4</td><td>-</td><td>-</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>6μl</td><td>6μl</td><td>6μl</td> |
</tr> | </tr> | ||
</table> | </table> | ||
:From left;<br> | :From left;<br> | ||
| - | :#BBa_R0010 | + | :#[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] |
:#Single Digestion | :#Single Digestion | ||
:#Double Digestion | :#Double Digestion | ||
| Line 82: | Line 82: | ||
:(26/8)--->'''[[Team:Chiba/protocol/PCR|Colony-PCR]]''' | :(26/8)--->'''[[Team:Chiba/protocol/PCR|Colony-PCR]]''' | ||
| - | ::Colony PCR of 5 colonies from ligation plates (26/8-(4),(5)) and one from control plate( | + | ::Colony PCR of 5 colonies from ligation plates (26/8-(4),(5)) and one from control plate([http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)). |
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
| Line 120: | Line 120: | ||
| - | : | + | :95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C |
| Line 140: | Line 140: | ||
<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>12μl</td></tr> |
</table> | </table> | ||
:From left;<br> | :From left;<br> | ||
| - | :1~5 insert: | + | :1~5 insert:[http://partsregistry.org/Part:BBa_C0170 BBa_C0170] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500] -> OK |
| - | :6 | + | :6 [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)-->Positive Control -> OK |
| - | :7~11 insert: | + | :7~11 insert:[http://partsregistry.org/Part:BBa_C0178 BBa_C0178] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500] -> OK |
|} | |} | ||
| - | |||
:--->(28/8)'''[[Team:Chiba/protocol/DNA Purification/sigma|Miniprep]]''' | :--->(28/8)'''[[Team:Chiba/protocol/DNA Purification/sigma|Miniprep]]''' | ||
| Line 172: | Line 171: | ||
:--->(29/8)'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' | :--->(29/8)'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' | ||
| - | |||
| - | |||
| - | |||
===Team:Output=== | ===Team:Output=== | ||
no work | no work | ||
Latest revision as of 20:14, 29 October 2008
26 August 2008 <|> 28 August 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
- Sample DNA :[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
| Sample No. | 1 | 2 | 3 |
| Sample DNA | 1 | 1 | 1 |
| PstI | - | - | 0.5 |
| EcoRI | - | 0.5 | 0.5 |
| Buffer 3 | - | 1 | 1 |
| BSA | - | - | 1 |
| dH2O | - | 7.5 | 2 |
| TOTAL | 1μl | 10μl | 10μl |
- --->Gel Check
|
|
- (26/8)--->Colony-PCR
- Colony PCR of 5 colonies from ligation plates (26/8-(4),(5)) and one from control plate([http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)).
DNA Template 1 dNTP mix 5 Foward Primer 5 Reverse Primer 5 DNA polymerase 0.5 Thermopol Buffer 5 dH2O 28.5 TOTAL 50μL
- 95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C
- --->Gel Check
Sample DNA 10 Loading Dye 2 TOTAL 12μl - From left;
- 1~5 insert:[http://partsregistry.org/Part:BBa_C0170 BBa_C0170] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500] -> OK
- 6 [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)-->Positive Control -> OK
- 7~11 insert:[http://partsregistry.org/Part:BBa_C0178 BBa_C0178] + vector:[http://partsregistry.org/Part:BBa_J04500 BBa_J04500] -> OK
- From left;
- --->(28/8)Miniprep
- competent cells : XL10G
- [http://partsregistry.org/Part:BBa_C0062 BBa_C0062](2007)
- [http://partsregistry.org/Part:BBa_C0062 BBa_C0062](2006) -> no colony on plates
- [http://partsregistry.org/Part:BBa_C0179 BBa_C0179](2007)
- [http://partsregistry.org/Part:BBa_C0179 BBa_C0179](2006)
- [http://partsregistry.org/Part:BBa_S03156 BBa_S03156](2007)
- [http://partsregistry.org/Part:BBa_S03156 BBa_S03156](2006)
- [http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2007)
- [http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2006)
- [http://partsregistry.org/Part:BBa_S03160 BBa_S03160](2007)
- [http://partsregistry.org/Part:BBa_S03160 BBa_S0316](2006)
- --->Mini prep
- --->(29/8)Gel Check
Team:Output
no work
