Team:Chiba/Calendar-Home/8 September 2008

From 2008.igem.org

(Difference between revisions)

Latest revision as of 23:43, 29 October 2008

>Home | Notebook

7 September 2008 <|> 9 September 2008

Laboratory work

Team:Input

no work

Team:Communication

-->(7/9)

Gel Extract

Sample DNA	60
Loading Dye	12
TOTAL	72μL

From left;

[http://partsregistry.org/Part:BBa_ BBa_](2007)

100 ng / 1 well

Sample Name	[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]	[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
Sample DNA	30	60
Loading Dye	6	12
TOTAL	36μL	72μL

From left;

[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]

[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]

Sample DNA	100
Loading Dye	20
TOTAL	6μL

[http://partsregistry.org/Part:BBa_C0261 BBa_C0261] -> none

--->Zymo Clean

eluted with 15μL(?) of NFW.

Delay Test

Team:Output

SAP

[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1)
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5)

Sample No.	1	5
DNA(digested sample)(μL)	7	6
SAP(μL)	2	2
SAP Buffer(μL)	3	3
TOTAL(μL)	30	30

-->left at 37 degrees for 1 hour.

-->left at 65 degrees for 30 minutes.

-->DNA purification(1:21μl,5:6μl)

-->Gel check

Sample No.	each
DNA(digested sample)(μL)	1
Loading Dye(μL)	1
dH₂O(μL)	4
TOTAL(μL)	6

vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1) 75ng/μl
insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2) 33.6ng/μl
insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](3) 33.6ng/μl
insert:[http://partsregistry.org/Part:BBa_S03160 BBa_S03160](4) 38.4ng/μl
vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5) 100ng/μl
insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](6) 33.6ng/μl

Sample No.	1&2	1&3	1&4	1	5&6	5
DNA(vector)(μL)	3	3	3	3	5	2.1
DNA(insert)(μL)	3	3	3	-	3.1	-
Ligase(μL)	1	1	1	1	1	1
Ligase Buffer(x5)(μL)	2	2	2	2	2	2
dH₂O(μL)	1	1	1	4	8.9	4
TOTAL(μL)	10	10	10	10	20	10

@@ Line 10: / Line 10: @@
 '''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
+{|align="justify"
+|[[Image:Chiba-0908.JPG|300px]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample DNA</td>
+<td>60</td>
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>12</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>72μL</td>
+</tr>
+</table>
+:From left;
+::[http://partsregistry.org/Part:BBa_ BBa_](2007)
+::100 ng / 1 well
+|}
+{|align="justify"
+|[[Image:Chiba-0908-2.JPG]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample Name</td>
+<td>[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]</td><td>[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]</td>
+</tr>
+<tr>
+<td width="257">Sample DNA</td>
+<td>30</td><td>60</td>
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>6</td><td>12</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>36μL</td><td>72μL</td>
+</tr>
+</table>
+:From left;
+::[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
+::[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
+|}
-:--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
+{|align="justify"
-:::->15μL?
+|[[Image:Chiba-0908-3.JPG]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample DNA</td>
+<td>100</td>
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>20</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>6μL</td>
+</tr>
+</table>
+::[http://partsregistry.org/Part:BBa_C0261 BBa_C0261] -> none
+|}
-===9 September, 2008===
--->(6/9)PCR of I9030
---->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
+:--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
+:::eluted with 15μL(?) of NFW.
---->'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
---->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
+'''[https://2008.igem.org/Team:Chiba/protocol/Time_Delay_Test|Time Delay Test]'''
 ===Team:Output===
 [[Team:Chiba/protocol/ligation/dephosphorylation|SAP]]
-*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)①
+*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1)
-*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤
+*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5)
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
 <td width="257">Sample No.</td>
-<td>①</td><td>⑤</td>
+<td>1</td><td>5</td>
 </tr>
 <tr>
-<td>DNA(digested sample)</td>
+<td>DNA(digested sample)(μL)</td>
 <td>7</td><td>6</td>
 </tr>
 <tr>
-<td>SAP/td>
+<td>SAP(μL)</td>
 <td>2</td><td>2</td>
 </tr>
 <tr>
-<td>SAP Buffer</td>
+<td>SAP Buffer(μL)</td>
 <td>3</td><td>3</td><td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
-<td>30</td><td>30</td>
-</tr>
-<tr>
-<td>TOTAL</td>
 <td>30</td><td>30</td>
 </tr>
 </table>
--->37℃ 1hour
+-->left at 37 degrees for 1 hour.
--->65℃ 30min
+-->left at 65 degrees for 30 minutes.
--->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]]
+-->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]](1:21μl,5:6μl)
--->21μl  6μl
 -->[[Team:Chiba/protocol/gelcheck|Gel check]]
@@ Line 75: / Line 129: @@
 </tr>
 <tr>
-<td>DNA(digested sample)</td>
+<td>DNA(digested sample)(μL)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>Loading Dye </td>
+<td>Loading Dye(μL)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>4</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>6</td>
 </tr>
 </table>
-*vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)① 75ng/μl
+*vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1) 75ng/μl
-*insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)② 33.6ng/μl
+*insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2) 33.6ng/μl
-*insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)③ 33.6ng/μl
+*insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](3) 33.6ng/μl
-*insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)④ 38.4ng/μl
+*insert:[http://partsregistry.org/Part:BBa_S03160 BBa_S03160](4) 38.4ng/μl
-*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤ 100ng/μl
+*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5) 100ng/μl
-*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](RBS+rLuc)⑥ 33.6ng/μl
+*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](6) 33.6ng/μl
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
 <td width="257">Sample No.</td>
-<td>①&②</td><td>①&③</td><td>①&④</td><td>①</td><td>⑤&⑥</td><td>⑤</td>
+<td>1&2</td><td>1&3</td><td>1&4</td><td>1</td><td>5&6</td><td>5</td>
 </tr>
 <tr>
-<td>DNA(vector)</td>
+<td>DNA(vector)(μL)</td>
 <td>3</td><td>3</td><td>3</td><td>3</td><td>5</td><td>2.1</td>
 </tr>
 <tr>
-<td>DNA(insert)</td>
+<td>DNA(insert)(μL)</td>
 <td>3</td><td>3</td><td>3</td><td>-</td><td>3.1</td><td>-</td>
 </tr>
 <tr>
-<td>Ligase</td>
+<td>Ligase(μL)</td>
 <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 </tr>
 <tr>
-<td>Ligase Buffer(x5)</td>
+<td>Ligase Buffer(x5)(μL)</td>
 <td>2</td><td>2</td><td>2</td><td>2</td><td>2</td><td>2</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>1</td><td>1</td><td>1</td><td>4</td><td>8.9</td><td>4</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>10</td><td>10</td><td>10</td><td>10</td><td>20</td><td>10</td>
 </tr>
 </table>