Team:Chiba/Calendar-Home/14 September 2008

From 2008.igem.org

(Difference between revisions)

Latest revision as of 23:59, 29 October 2008

>Home | Notebook

13 September 2008 <|> 15 September 2008

Laboratory work

Team:Communication

(13/9)--->Mini prep

[http://partsregistry.org/Part:BBa_I7106 BBa_I7106]
[http://partsregistry.org/Part:BBa_I23019 BBa_I23019]

(13/9)--->[[1]]

[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]

--->(19/9)

Transformation

Competent cells : XL10Gold

[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084009 BBa_K084009](Plac+RhlI+LVA)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA)

--->(19/9)Gel Check

Time Delay Test

Team:Output

Time Responce Liquid

Digestion test

[http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510]
[http://partsregistry.org/Part:BBa_I716463 BBa_I716463]
[http://partsregistry.org/Part:BBa_C0051 BBa_C0051]
[http://partsregistry.org/Part:BBa_C0070 BBa_C0070]
[http://partsregistry.org/Part:BBa_J06620 BBa_J06620]
[http://partsregistry.org/Part:BBa_J22141 BBa_J22141]
[http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
[http://partsregistry.org/Part:BBa_I716452 BBa_I716452]
[http://partsregistry.org/Part:BBa_I716462 BBa_I716462]
[http://partsregistry.org/Part:BBa_C0078 BBa_C0078]
[http://partsregistry.org/Part:BBa_J01101 BBa_J01101]
[http://partsregistry.org/Part:BBa_C0071 BBa_C0071]
[http://partsregistry.org/Part:BBa_I712019 BBa_I712019]
[http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
[http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
[http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
[http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
[http://partsregistry.org/Part:BBa_I7100 BBa_I7100](×2)
[http://partsregistry.org/Part:BBa_S01416 BBa_S01416]

Sample No.	each
DNA(digested sample)(μL)	3
Buffer2(x10)(μL)	1
dH₂O(μL)	6
EcoRI(μL)	0.075
TOTAL(μL)	10

--> left at 37°C for 1 hour

Gel check

Sample No.	each
DNA(digestion sample)(μL)	10
Loading Dye(μL)	2
TOTAL(μL)	12

@@ Line 5: / Line 5: @@
 ==Laboratory work==
 ===Team:Communication===
-:(13/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
+(13/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
+::*[http://partsregistry.org/Part:BBa_I7106 BBa_I7106]
+::*[http://partsregistry.org/Part:BBa_I23019 BBa_I23019]
+(13/9)--->[[https://2008.igem.org/Team:Chiba/protocol/ligation/dephosphorylation|SAP]]
+::*[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
+::*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
+:--->(19/9)
+'''[[Team:Chiba/protocol/transformation|Transformation]]'''
+:Competent cells : XL10Gold
+::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)
+::*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084009 BBa_K084009](Plac+RhlI+LVA)
+::*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA)
+:--->(19/9)'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
+'''[[Team:Chiba/protocol/Time Delay Test|Time Delay Test]]'''
-:'''[[Team:Chiba/protocol/transformation|Transformation]]'''
-:Competent cells : XL10G
-::*BBa_T9002[http://partsregistry.org/Part:BBa_T9002](2007)
-::*Plac+RhlI+LVA
-::*Plac+CinI+LVA
-:--->(15/9)
 ===Team:Output===
-<br>[[Team:Chiba/jk/γ/Trl|Time Responce Liquid]]
-<br>Digestion test
+[[Team:Chiba/jk/γ/Trl|Time Responce Liquid]]
+[[Team:Chiba/protocol/digestion|Digestion test]]
 *[http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510]
 *[http://partsregistry.org/Part:BBa_I716463 BBa_I716463]
@@ Line 28: / Line 43: @@
 *[http://partsregistry.org/Part:BBa_J22141 BBa_J22141]
 *[http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
-*BBa_I716542
+*[http://partsregistry.org/Part:BBa_I716452 BBa_I716452]
 *[http://partsregistry.org/Part:BBa_I716462 BBa_I716462]
 *[http://partsregistry.org/Part:BBa_C0078 BBa_C0078]
@@ Line 38: / Line 53: @@
 *[http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
 *[http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
-*[http://partsregistry.org/Part:BBa_I7100 BBa_I7100](x2)
+*[http://partsregistry.org/Part:BBa_I7100 BBa_I7100](&times;2)
 *[http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>each</td>
 </tr>
 <tr>
-<td>DNA(digested sample)</td>
+<td>DNA(digested sample)(&mu;L)</td>
 <td>3</td>
 </tr>
 <tr>
-<td>Buffer2(x10)</td>
+<td>Buffer2(x10)(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>6</td>
 </tr>
 <tr>
-<td>EcoRⅠ</td>
+<td>EcoRI(&mu;L)</td>
 <td>0.075</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(&mu;L)</td>
 <td>10</td>
 </tr>
 </table>
--->37℃ 1hour
+--> left at 37&deg;C for 1 hour
 [[Team:Chiba/protocol/gelcheck|Gel check]]
@@ Line 73: / Line 89: @@
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>each</td>
 </tr>
 <tr>
-<td>DNA(digestion sample)</td>
+<td>DNA(digestion sample)(&mu;L)</td>
 <td>10</td>
 </tr>
 <tr>
-<td>Loading Dye</td>
+<td>Loading Dye(&mu;L)</td>
 <td>2</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(&mu;L)</td>
 <td>12</td>
 </tr>
 </table>