Team:Chiba/Calendar-Home/18 September 2008

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===Team:Communication===
===Team:Communication===
*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
-
**conditions
+
**transformants
-
#strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
+
***[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
-
#temparature<br>37℃ and 30℃
+
***[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
 +
***[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
 +
**E.coli strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
 +
**temparature<br>37&deg;C  and 30&deg;C
===Team:Output===
===Team:Output===
-
<br>SAP 1μl
+
[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]] 1&mu;L
-
<br>->37℃　1hour
+
 
-
<br>->30min　65℃
+
->left for:
-
<br>Zymo ->25μl
+
#1 hour at 37 &deg;C
-
<br>Ligation
+
#30 minutes at 65 &deg;C
-
<br>vector:BBa_I7100
+
 
-
<br>insert:BBa_S03119(ptet+LuxR),Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR)
+
[[Team:Chiba/protocol/transformation|Zymoclean]]
 +
 
 +
->eluted with 25&mu;L of NFW.
 +
 
 +
[[Team:Chiba/protocol/ligation/ligation|Ligation]]
 +
*vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
 +
*insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119]
 +
*insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084004 K084004]
 +
*insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084005 K084005]
 +
*insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084006 K084006]
 +
 
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
<td>Vector&insert</td><td>Vector</td>
<td>Vector&insert</td><td>Vector</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(vector)</td>
+
<td>DNA(vector)(&mu;L)</td>
<td>3.5</td><td>3.5</td>
<td>3.5</td><td>3.5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(insert)</td>
+
<td>DNA(insert)(&mu;L)</td>
<td>7.5</td><td>-</td>
<td>7.5</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Ligase</td>
+
<td>Ligase(&mu;L)</td>
<td>1</td><td>1</td>
<td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Ligase Buffer</td>
+
<td>Ligase Buffer(&mu;L)</td>
<td>4</td><td>4</td>
<td>4</td><td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>4</td><td>4</td>
<td>4</td><td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>20</td><td>20</td>
<td>20</td><td>20</td>
</tr>
</tr>
</table>
</table>
-
<br>TRANSFORMATION
+
[[Team:Chiba/protocol/transformation|transformation]]
-
<br>BBa_J54103(RBS+GFP)
+
*[http://partsregistry.org/Part:BBa_J54103 BBa_J54103]
-
<br>BBa_P2006
+
*[http://partsregistry.org/Part:BBa_P2006 BBa_P2006]
[[Team:Chiba/protocol/gelcheck|Gel check]]
[[Team:Chiba/protocol/gelcheck|Gel check]]
-
<br>Ligation product(Venus YFP)
+
*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003]
-
<br>BBa_J37033
+
*[http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
-
<br>BBa_J37020
+
*[http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
-
<br>BBa_J37032
+
*[http://partsregistry.org/Part:BBa_J37032 BBa_J37032]
 +
 
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
<td>each</td>
<td>each</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA</td>
+
<td>DNA(&mu;L)</td>
<td>each</td>
<td>each</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>4</td>
<td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>6</td>
<td>6</td>
</tr>
</tr>
</table>
</table>

Latest revision as of 00:01, 30 October 2008

>Home | Notebook

17 September 2008 <|> 19 September 2008

Laboratory work

Team:Communication

  • time-delay test
    • transformants
      • [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
      • [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
      • [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
    • E.coli strain
      Senders:XL10GOLD
      Receiver:JW1908
    • temparature
      37°C and 30°C


Team:Output

SAP 1μL

->left for:

  1. 1 hour at 37 °C
  2. 30 minutes at 65 °C

Zymoclean

->eluted with 25μL of NFW.

Ligation

  • vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
  • insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119]
  • insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084004 K084004]
  • insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084005 K084005]
  • insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084006 K084006]
Sample No. Vector&insertVector
DNA(vector)(μL) 3.53.5
DNA(insert)(μL) 7.5-
Ligase(μL) 11
Ligase Buffer(μL) 44
dH2O(μL) 44
TOTAL(μL) 2020

transformation

  • [http://partsregistry.org/Part:BBa_J54103 BBa_J54103]
  • [http://partsregistry.org/Part:BBa_P2006 BBa_P2006]

Gel check

  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003]
  • [http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
  • [http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
  • [http://partsregistry.org/Part:BBa_J37032 BBa_J37032]
Sample No. each
DNA(μL) each
Loading Dye(μL) 1
dH2O(μL) 4
TOTAL(μL) 6
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