Team:Chiba/Calendar-Home/20 September 2008

From 2008.igem.org

(Difference between revisions)
(→Laboratory work)
(→Laboratory work)
 
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Line 9: Line 9:
--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
-
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
{|align="justify"
 +
|[[Image:Chiba-0920.JPG|300px]]
 +
|<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
<td width="257">Sample Name</td>
<td width="257">Sample Name</td>
-
<td>Lagation(J04500+C0161)</td>
+
<td>Lagation([http://partsregistry.org/Part:BBa_J04500 BBa_J04500]+[http://partsregistry.org/Part:BBa_C0161 BBa_C0161])</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Sample DNA</td>
+
<td>Sample DNA(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>H<sub>2</sub>O</td>
+
<td>H<sub>2</sub>O(&mu;L)</td>
<td>4</td>
<td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>6</td>
<td>6</td>
</tr>
</tr>
Line 36: Line 38:
::#　-> OK
::#　-> OK
::#　-> OK
::#　-> OK
 +
|}
:--->'''[[Team:Chiba/protocol/digestion|Digestion Test]]'''
:--->'''[[Team:Chiba/protocol/digestion|Digestion Test]]'''
Line 44: Line 47:
</tr>
</tr>
<tr>
<tr>
-
<td>Sample DNA</td>
+
<td>Sample DNA(&mu;L)</td>
<td>3</td><td>3</td>
<td>3</td><td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>XbaⅠ</td>
+
<td>XbaI</td>
<td>0.25?</td><td>0.25</td>
<td>0.25?</td><td>0.25</td>
</tr>
</tr>
<tr>
<tr>
-
<td>SpeⅠ</td>
+
<td>SpeI</td>
-
<td>0.5?</td><td>0.5</td>
+
<td>0.5</td><td>0.5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer 2</td>
+
<td>Buffer 2(&mu;L)</td>
<td>2</td><td>2</td>
<td>2</td><td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BSA</td>
+
<td>BSA(&mu;L)</td>
-
<td>2?</td><td>2?</td>
+
<td>2</td><td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>?</td><td>12.5</td>
<td>?</td><td>12.5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>20</td><td>20</td>
<td>20</td><td>20</td>
</tr>
</tr>
Line 76: Line 79:
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
{|align="justify"
 +
|[[Image:Chiba-0920-2.JPG|300px]]
 +
|:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
Line 82: Line 87:
</tr>
</tr>
<tr>
<tr>
-
<td>Sample DNA</td>
+
<td>Sample DNA(&mu;L)</td>
<td>20</td><td>20</td>
<td>20</td><td>20</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(&mu;L)</td>
<td>4</td><td>4</td>
<td>4</td><td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>24</td><td>24</td>
<td>24</td><td>24</td>
</tr>
</tr>
Line 103: Line 108:
::#　-> OK
::#　-> OK
::#　-> OK
::#　-> OK
 +
|}
--->(?/9)'''[[Team:Chiba/protocol/transformation|Transformation]]'''
--->(?/9)'''[[Team:Chiba/protocol/transformation|Transformation]]'''
--->(22/9)'''phenotype check
--->(22/9)'''phenotype check
 +
 +
===Team:Output===
===Team:Output===
no work
no work

Latest revision as of 00:25, 30 October 2008

>Home | Notebook

19 September 2008 <|> 21 September 2008

Laboratory work

Team:Communication

--->(19/9)Mini prep

--->Gel Check

Chiba-0920.JPG
Sample Name Lagation([http://partsregistry.org/Part:BBa_J04500 BBa_J04500]+[http://partsregistry.org/Part:BBa_C0161 BBa_C0161])
Sample DNA(μL) 1
Loading Dye(μL) 1
H2O(μL) 4
TOTAL(μL) 6
From left;
  1. 　-> OK
  2. 　-> OK
  3. 　-> OK
  4. 　-> OK
--->Digestion Test
Sample No. 1-45-8
Sample DNA(μL) 33
XbaI 0.25?0.25
SpeI 0.50.5
Buffer 2(μL) 22
BSA(μL) 22
dH2O(μL) ?12.5
TOTAL(μL) 2020


--->Gel Check
Chiba-0920-2.JPG :
Sample No. 1-45~8
Sample DNA(μL) 2020
Loading Dye(μL) 44
TOTAL(μL) 2424
From left;
  1. 　-> OK
  2. 　-> OK
  3. 　-> OK
  4. 　-> OK
  5. 　-> OK
  6. 　-> OK
  7. 　-> OK
  8. 　-> OK

--->(?/9)Transformation

--->(22/9)phenotype check



Team:Output

no work

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/20_September_2008"