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Team:Chiba/Calendar-Home/26 September 2008
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===Team:Output=== | ===Team:Output=== | ||
| - | + | <br>colony [[Team:Chiba/protocol/PCR|PCR]] | |
| - | + | <br>Vector:[http://partsregistry.org/Part:BBa_R0062 BBa_R0062] Insert:[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]+[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]×7 | |
| - | + | ||
| - | <br>colony PCR | + | |
| - | + | ||
| - | <br>Vector:BBa_R0062 | + | |
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
<tr> | <tr> | ||
| - | <td width="257">Sample No</td> | + | <td width="257">Sample No.</td> |
<td>each</td> | <td>each</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>culture</td> | + | <td>culture(μL)</td> |
<td>1</td> | <td>1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Fwd primer</td> | + | <td>Fwd primer(μL)</td> |
<td>1.5</td> | <td>1.5</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Rev primer</td> | + | <td>Rev primer(μL)</td> |
<td>1.5</td> | <td>1.5</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>Taq(DNA pol)</td> | + | <td>Taq(DNA pol)(μL)</td> |
<td>0.2</td> | <td>0.2</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>dNTPmix</td> | + | <td>dNTPmix(μL)</td> |
<td>3</td> | <td>3</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>thermo pol Buffer</td> | + | <td>thermo pol Buffer(μL)</td> |
<td>3</td> | <td>3</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>dH<sub>2</sub>O(μL)</td> |
<td>19.8</td> | <td>19.8</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td>TOTAL</td> | + | <td>TOTAL(μL)</td> |
<td>30</td> | <td>30</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
| - | <br>-> | + | <br>->95°C 5 min ->(95°C 1 min 50°C 30 sec 72°C 1 min)×25 ->72°C 10 min |
| - | <br>->Gel | + | <br>->[[Team:Chiba/protocol/gelcheck|Gel check]] |
| - | <br>->MINI Prep | + | <br>->[[Team:Chiba/protocol/DNA Purification/sigma|MINI Prep]] |
Latest revision as of 01:08, 30 October 2008
25 September 2008 <|> 27 September 2008
Laboratory work
Team:Communication
Team:Output
colony PCR
Vector:[http://partsregistry.org/Part:BBa_R0062 BBa_R0062] Insert:[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]+[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]×7
| Sample No. | each |
| culture(μL) | 1 |
| Fwd primer(μL) | 1.5 |
| Rev primer(μL) | 1.5 |
| Taq(DNA pol)(μL) | 0.2 |
| dNTPmix(μL) | 3 |
| thermo pol Buffer(μL) | 3 |
| dH2O(μL) | 19.8 |
| TOTAL(μL) | 30 |
->95°C 5 min ->(95°C 1 min 50°C 30 sec 72°C 1 min)×25 ->72°C 10 min
->Gel check
->MINI Prep
