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Team:Warsaw/Calendar-Main/4 July 2008
From 2008.igem.org
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| - | <h3> | + | <h3>Change of the reporter from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with B-galactosidase to GFP or RFP</h3> |
| - | <h4>Piotr, Weronika</h4> | + | <h4>Piotr, Weronika, Emilia</h4> |
<ol> | <ol> | ||
<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from transformants.</li> | <li><a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from transformants.</li> | ||
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest"> digest</a> of plasmids with NotI.</li> | <li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest"> digest</a> of plasmids with NotI.</li> | ||
| - | <li>Gel electrophoresis of digested DNA | + | <li>Gel electrophoresis of digested DNA. There where proper clones, but they weren't red or green in UV (the reason might be low copy number of the plasmid or something else).</li> |
</li></ol> | </li></ol> | ||
| - | No sucess in changing of the reporter from | + | <p>No sucess in changing of the reporter from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with B-galactosidase to GFP or RFP.</p> |
| - | <h3>Preparation of constructs | + | <h3>Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega</h3> |
<h4>Paweł</h4> | <h4>Paweł</h4> | ||
<p><ol> | <p><ol> | ||
Latest revision as of 14:07, 28 October 2008
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Change of the reporter from pZC320 with B-galactosidase to GFP or RFPPiotr, Weronika, Emilia
No sucess in changing of the reporter from pZC320 with B-galactosidase to GFP or RFP. Preparation of constructs on pET15b: OmpA_alpha and OmpA_omegaPaweł
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