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Team:Warsaw/Calendar-Main/2 July 2008
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<h3>Change of the reporter from <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with B-galactosidase to GFP or RFP</h3> | <h3>Change of the reporter from <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with B-galactosidase to GFP or RFP</h3> | ||
| - | <h4>Piotr, Weronika</h4> | + | <h4>Piotr, Weronika, Emilia</h4> |
<ol> | <ol> | ||
<li> | <li> | ||
| - | Gel electrophoresis and gel-out of proper bands | + | Gel electrophoresis and gel-out of proper bands. |
</li> | </li> | ||
<li> | <li> | ||
| - | Ligation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with standard parts: <A href=http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840>BBa_E0840</a>( | + | Ligation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a> with standard parts: <A href=http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840>BBa_E0840</a>(GFP genetrator) and <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450>BBa_J04450</a> (RFP generator). |
</li> | </li> | ||
| - | <li>Chemotransformation of E.coli TOP10 with ligation products</li> | + | <li>Chemotransformation of E.coli TOP10 with ligation products.</li> |
| - | <li>Plating transformants on LB+Amp30+X-gal+IPTG | + | <li>Plating transformants on LB+Amp30+X-gal+IPTG.</li></ol></p> |
| - | <h3>Preparation of constructs | + | <h3>Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega</h3> |
<h4>Paweł</h4> | <h4>Paweł</h4> | ||
<p><ol> | <p><ol> | ||
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up</a> of overnight digest.</li> | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up</a> of overnight digest.</li> | ||
| - | <li> <A HREF="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of | + | <li> <A HREF="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of OmpA_alpha (from Michał K.) and <a href=http://www.emdbiosciences.com/docs/docs/PROT/TB045.pdf>pET15b</a>. </li> |
| - | <li> <A HREF="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of OmpA_omega and <a href=http://www.emdbiosciences.com/docs/docs/PROT/TB045.pdf>pET15b</a>.</li> | + | <li> <A HREF="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of OmpA_omega (from Michał K.) and <a href=http://www.emdbiosciences.com/docs/docs/PROT/TB045.pdf>pET15b</a>.</li> |
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htmtop10">TOP10</a> strain with ligation products. </li> | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htmtop10">TOP10</a> strain with ligation products. </li> | ||
<li> Transformants plating on LB + ampicillin.</li></ol> | <li> Transformants plating on LB + ampicillin.</li></ol> | ||
Latest revision as of 14:05, 28 October 2008
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Change of the reporter from pZC320 with B-galactosidase to GFP or RFPPiotr, Weronika, Emilia
Preparation of constructs on pET15b: OmpA_alpha and OmpA_omegaPaweł
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