Cloning omega-A fusion on pKS (second attempt)

Michał L., Ewa, Marcin

Sequencing confirms that we have obtained construct with omega-A fusion, but A in our constructs turned out to be truncated and contain only one B-domain (which we called 'delta A'). We inoculated bacteria containing this plasmid to obtain it for futher cloning.

Purification of proteins: Z-alpha and Z-omega

Piotr

Single transformations of Rosetta with plasmids pET15b+Z-alpha and pET15b+Z-omega. Plating them on LB with chloramphenicol and ampicillin.