Team:NTU-Singapore/Parts/Fluorescence Microscope Image

From 2008.igem.org

(Difference between revisions)
(→pLacI-GFP part:BBa_J04430 found to express RFP instead)
 
(2 intermediate revisions not shown)
Line 4: Line 4:
<div id="maincontent" style="margin-top:200px;">
<div id="maincontent" style="margin-top:200px;">
-
=pLacI-GFP part:BBa_J04430 found to express RFP instead=
+
='''Images and Videos from the Characterization Lab'''=
 +
=='''pLacI-GFP part:BBa_J04430 found to express RFP instead'''==
The below pictures were taken after the BBa_J04430 was transformed into Top 10 competent cells. They clearly showed, either under normal light or UV light, the cells appears red or glow red respectively. This is contrary to what was expected, as we would expect it to glow green under UV light. This problem was highlighted to the iGEM Headquarters. In order to solve this problem the pLac promoter (R0010) was ligated with the GFP (E0040), allowing us to proceed further with the characterization experiments.
The below pictures were taken after the BBa_J04430 was transformed into Top 10 competent cells. They clearly showed, either under normal light or UV light, the cells appears red or glow red respectively. This is contrary to what was expected, as we would expect it to glow green under UV light. This problem was highlighted to the iGEM Headquarters. In order to solve this problem the pLac promoter (R0010) was ligated with the GFP (E0040), allowing us to proceed further with the characterization experiments.
Line 12: Line 13:
|}
|}
-
=Image results obtained from fluorescence microscope=
+
=='''Image results obtained from fluorescence microscope'''==
Below are Images obtained from the microscope of the various standards (Low, Medium, High & Standard) that iGEM Headquarters has provided for us in the [http://partsregistry.org/Measurement/SPU/Learn iGEM Newsletter].  
Below are Images obtained from the microscope of the various standards (Low, Medium, High & Standard) that iGEM Headquarters has provided for us in the [http://partsregistry.org/Measurement/SPU/Learn iGEM Newsletter].  
-
==Weak promoter [http://partsregistry.org/Part:BBa_J23150 BBa_J23150]==
+
===Weak promoter [http://partsregistry.org/Part:BBa_J23150 BBa_J23150]===
{| align="center" border="1"
{| align="center" border="1"
|[[Image:102-norm_030708.JPG|thumb|400px|BBa_J23150 Before Excitation]]
|[[Image:102-norm_030708.JPG|thumb|400px|BBa_J23150 Before Excitation]]
Line 21: Line 22:
|}
|}
-
==Medium promoter [http://partsregistry.org/Part:BBa_J23151 BBa_J23151]==
+
===Medium promoter [http://partsregistry.org/Part:BBa_J23151 BBa_J23151]===
{| align="center" border="1"
{| align="center" border="1"
|[[Image: 150_norm_030708.JPG|thumb|400px|BBa_J23151 Before Excitation ]]
|[[Image: 150_norm_030708.JPG|thumb|400px|BBa_J23151 Before Excitation ]]
Line 27: Line 28:
|}
|}
-
==Strong promoter [http://partsregistry.org/Part:BBa_J23102 BBa_J23102] ==
+
===Strong promoter [http://partsregistry.org/Part:BBa_J23102 BBa_J23102] ===
{| align="center" border="1"
{| align="center" border="1"
|[[Image: 151_norm_030708.JPG|thumb|400px|BBa_J23102 Before Excitation]]
|[[Image: 151_norm_030708.JPG|thumb|400px|BBa_J23102 Before Excitation]]
Line 33: Line 34:
|}
|}
-
==Standard promoter with GFP reporter [http://partsregistry.org/Part:BBa_I20260 BBa_I20260]  ==
+
===Standard promoter with GFP reporter [http://partsregistry.org/Part:BBa_I20260 BBa_I20260]  ===
{| align="center" border="1"
{| align="center" border="1"
|[[Image:260norm_030708.JPG|thumb|400px|BBa_I20260Before Excita]]
|[[Image:260norm_030708.JPG|thumb|400px|BBa_I20260Before Excita]]
Line 48: Line 49:
<html><object width="425" height="344"><param name="movie" value="http://www.youtube.com/v/-NlpYG2YTZM&hl=en&fs=1"></param><param name="allowFullScreen" value="true"></param><embed src="http://www.youtube.com/v/-NlpYG2YTZM&hl=en&fs=1" type="application/x-shockwave-flash" allowfullscreen="true" width="425" height="344"></embed></object></html> <br>
<html><object width="425" height="344"><param name="movie" value="http://www.youtube.com/v/-NlpYG2YTZM&hl=en&fs=1"></param><param name="allowFullScreen" value="true"></param><embed src="http://www.youtube.com/v/-NlpYG2YTZM&hl=en&fs=1" type="application/x-shockwave-flash" allowfullscreen="true" width="425" height="344"></embed></object></html> <br>
Video of E coli under blue light excitation
Video of E coli under blue light excitation
 +
<br><br>
 +
<html>
 +
<script language=Javascript1.2>
 +
<!--
 +
 +
var tags_before_clock = "<b>It is now "
 +
var tags_middle_clock = "on"
 +
var tags_after_clock  = "</b>"
 +
 +
if(navigator.appName == "Netscape") {
 +
document.write('<layer id="clock"></layer><br>');
 +
}
 +
 +
if (navigator.appVersion.indexOf("MSIE") != -1){
 +
document.write('<span id="clock"></span>');
 +
}
 +
 +
DaysofWeek = new Array()
 +
  DaysofWeek[0]="Sunday"
 +
  DaysofWeek[1]="Monday"
 +
  DaysofWeek[2]="Tuesday"
 +
  DaysofWeek[3]="Wednesday"
 +
  DaysofWeek[4]="Thursday"
 +
  DaysofWeek[5]="Friday"
 +
  DaysofWeek[6]="Saturday"
 +
 +
Months = new Array()
 +
  Months[0]="January"
 +
  Months[1]="February"
 +
  Months[2]="March"
 +
  Months[3]="April"
 +
  Months[4]="May"
 +
  Months[5]="June"
 +
  Months[6]="July"
 +
  Months[7]="August"
 +
  Months[8]="September"
 +
  Months[9]="October"
 +
  Months[10]="November"
 +
  Months[11]="December"
 +
 +
function upclock(){
 +
var dte = new Date();
 +
var hrs = dte.getHours();
 +
var min = dte.getMinutes();
 +
var sec = dte.getSeconds();
 +
var day = DaysofWeek[dte.getDay()]
 +
var date = dte.getDate()
 +
var month = Months[dte.getMonth()]
 +
var year = dte.getFullYear()
 +
 +
var col = ":";
 +
var spc = " ";
 +
var com = ",";
 +
var apm;
 +
 +
if (date == 1 || date == 21 || date == 31)
 +
  {ender = "<sup>st</sup>"}
 +
else
 +
if (date == 2 || date == 22)
 +
  {ender = "<sup>nd</sup>"}
 +
else
 +
if (date == 3 || date == 23)
 +
  {ender = "<sup>rd</sup>"}
 +
 +
else
 +
  {ender = "<sup>th</sup>"}
 +
 +
if (12 < hrs) {
 +
apm="<font size='-1'>pm</font>";
 +
hrs-=12;
 +
}
 +
 +
else {
 +
apm="<font size='-1'>am</font>";
 +
}
 +
 +
if (hrs == 0) hrs=12;
 +
if (hrs<=9) hrs="0"+hrs;
 +
if (min<=9) min="0"+min;
 +
if (sec<=9) sec="0"+sec;
 +
 +
if(navigator.appName == "Netscape") {
 +
document.clock.document.write(tags_before_clock+hrs+col+min+col+sec+apm+spc+tags_middle_clock+spc+day+com+spc+date+ender+spc+month+com+spc+year+tags_after_clock);
 +
document.clock.document.close();
 +
}
 +
 +
if (navigator.appVersion.indexOf("MSIE") != -1){
 +
clock.innerHTML = tags_before_clock+hrs+col+min+col+sec+apm+spc+tags_middle_clock+spc+day+com+spc+date+ender+spc+month+com+spc+year+tags_after_clock;
 +
}
 +
}
 +
 +
setInterval("upclock()",1000);
 +
//-->
 +
</script>
 +
</html>

Latest revision as of 05:50, 27 October 2008

Contents

Images and Videos from the Characterization Lab

pLacI-GFP part:BBa_J04430 found to express RFP instead

The below pictures were taken after the BBa_J04430 was transformed into Top 10 competent cells. They clearly showed, either under normal light or UV light, the cells appears red or glow red respectively. This is contrary to what was expected, as we would expect it to glow green under UV light. This problem was highlighted to the iGEM Headquarters. In order to solve this problem the pLac promoter (R0010) was ligated with the GFP (E0040), allowing us to proceed further with the characterization experiments.

Cells appears pinkish even under normal light
Cells glow PINK under UV light

Image results obtained from fluorescence microscope

Below are Images obtained from the microscope of the various standards (Low, Medium, High & Standard) that iGEM Headquarters has provided for us in the [http://partsregistry.org/Measurement/SPU/Learn iGEM Newsletter].

Weak promoter [http://partsregistry.org/Part:BBa_J23150 BBa_J23150]

BBa_J23150 Before Excitation
BBa_J23150 After Excitation

Medium promoter [http://partsregistry.org/Part:BBa_J23151 BBa_J23151]

BBa_J23151 Before Excitation
BBa_J23151 After Excitation

Strong promoter [http://partsregistry.org/Part:BBa_J23102 BBa_J23102]

BBa_J23102 Before Excitation
BBa_J23102 After Excitation

Standard promoter with GFP reporter [http://partsregistry.org/Part:BBa_I20260 BBa_I20260]

BBa_I20260Before Excita
BBa_I20260After Excitation

The above pictures showed the green fluorescence protein was produced by Top 10 competent cells that were taken under blue light excitation. These cells contained the different standard promoters with GFP and they have been allowed to grow to an OD of 1.2 before a snap shot was taken with the microscope.

Video obtained from fluorescence microscope


Video of E coli under normal white light



Video of E coli under blue light excitation

Retrieved from "http://2008.igem.org/Team:NTU-Singapore/Parts/Fluorescence_Microscope_Image"