Team:Warsaw/Calendar-Main/19 September 2008

From 2008.igem.org

(Difference between revisions)
 
(10 intermediate revisions not shown)
Line 5: Line 5:
<html>
<html>
-
<h3>Preparation of BioBricks</h3>
 
-
<h4>Michał K.</h4>
 
-
<ol>
 
-
<li> Gel electrophoresis to estimate concentration of purified DNA from previous day. </li>
 
-
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments of RFP(??????) and deltaA. </li></ol>
 
-
<h3></h3>
+
<h3>MutD<sub>5</sub> testing</h3>
<h4>Piotr</h4>
<h4>Piotr</h4>
-
<ol><li> <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">transformation</a> with the above ligation. </li>
+
<p>Testing MutD<sub>5</sub> ampicillin resistance - MutD<sub>5</sub> isn't resistant to ampicillin</p>
 +
 
 +
 
 +
 
 +
 
 +
<h3>'Hunter and prey' system tests: Competition tests</h3>
 +
<h4>Emilia</h4>
 +
 
 +
 
 +
<ol><li><p>Samples of LB (0.25 mM IPTG; ampicillin 50 μl/ml) was inoculated with following strain combinations:</li>
 +
<ul><li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a> - A_alpha protein added ~100 μg </li>
 +
<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a> - Z_alpha protein added ~100 μg</li>
 +
<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-alpha>pACYC177+OmpA_A_alpha</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a> - Z_omega protein added ~100 μg</li> </ul></p>
 +
</li><li>Bacteria centrifuged and frozen
 +
</li></ol>
 +
 
 +
 
 +
<h3>Preparation of <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_K103003>&Delta;A (BBa_K103003)</a></h3>
 +
<h4>Michał K., Piotr</h4>
 +
<ol>
 +
<li> Gel electrophoresis to estimate concentration of purified DNA from previous day. </li>
 +
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragments of <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a> and &Delta;A. </li><li> <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">transformation</a> with the above ligation. </li>
<li>Plating on LB + ampicillin.</li>
<li>Plating on LB + ampicillin.</li>

Latest revision as of 21:14, 29 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 



MutD5 testing

Piotr

Testing MutD5 ampicillin resistance - MutD5 isn't resistant to ampicillin

'Hunter and prey' system tests: Competition tests

Emilia

  1. Samples of LB (0.25 mM IPTG; ampicillin 50 μl/ml) was inoculated with following strain combinations:

  2. Bacteria centrifuged and frozen

Preparation of ΔA (BBa_K103003)

Michał K., Piotr

  1. Gel electrophoresis to estimate concentration of purified DNA from previous day.
  2. Ligation of isolated DNA fragments of pSB1A3 and ΔA.
  3. E. coli TOP10 transformation with the above ligation.
  4. Plating on LB + ampicillin.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/19_September_2008"