Team:Warsaw/Calendar-Main/11 September 2008

From 2008.igem.org

(Difference between revisions)
 
(10 intermediate revisions not shown)
Line 7: Line 7:
-
<p><ol><li>Inoculation of OmpA_variant from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicylin with minimum concentration of prey (yesterday results).</li>
+
<p><ol><li>Inoculation of pACYC177+OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicillin with minimum concentration of prey (yesterday's results).</li>
-
<li>Mesurment of growth after 6 hours - 2 fold digger growth with interacting proteins (Omp_A_omega twice as more as Omp_omega with protein Z_alpha etc..)!!!
+
<li>Measurement of growth after 6 hours - 2-fold growth increase in presence of interacting proteins (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a> twice as much as <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> with protein Z_alpha, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-omega>pACYC177+OmpA_Z_omega</a> twice as much as
 +
<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega </a>with protein A_alpha and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-Z-alpha>pACYC177+OmpA_Z_alpha</a> twice as much as <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> with protein Z_omega.)!!!!</li></ol></p>
-
<br><br><br>
 
-
We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</p><br><br>
 
 +
<h3>We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</h3>
 +
<h4></h4>

Latest revision as of 12:26, 29 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Tests for ampicillin resistance given by protein added to medium

Piotr

  1. Inoculation of pACYC177+OmpA_variants from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicillin with minimum concentration of prey (yesterday's results).
  2. Measurement of growth after 6 hours - 2-fold growth increase in presence of interacting proteins (pACYC177+OmpA_A_omega twice as much as pACYC177+OmpA_omega with protein Z_alpha, pACYC177+OmpA_Z_omega twice as much as pACYC177+OmpA_omega with protein A_alpha and pACYC177+OmpA_Z_alpha twice as much as pACYC177+OmpA_alpha with protein Z_omega.)!!!!

We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/11_September_2008"