Team:NTU-Singapore/Modelling/Sensitivity Analysis

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<div id="maincontent" style="margin-top:200px;">
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=Sensitivity Analysis=
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='''Sensitivity Analysis'''=
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A sensitivty analysis for the two systems were performed using [http://www.sbtoolbox2.org/main.php SBtoolbox 2]. The reason for performing this analysis is to find out which are the important parameters and would have a major effects on the experimental results. A part of the reason is because some of the parameters were fitted and not obtained strictly from literature. This analysis would allow us to plan for experiments while taking into account the effect these parameters may have.
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Sensitivty analyses for the two systems were performed using [http://www.sbtoolbox2.org/main.php SBtoolbox 2]. The reason for performing this analysis is to find out which are the important parameters and would have a major effects on the experimental results. A part of the reason is because some of the parameters were fitted and not obtained strictly from literature. This analysis would allow us to plan for experiments while taking into account the effect these parameters may have.
==E7 production system Sensitivity Analysis==
==E7 production system Sensitivity Analysis==
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We can compare the new adjusted E7 production system with the [https://2008.igem.org/Team:NTU-Singapore/Modelling/Deterministic_Modeling#E7_production_system previous modeling exercise]. The E7 and immunity protein production has been cut down.
We can compare the new adjusted E7 production system with the [https://2008.igem.org/Team:NTU-Singapore/Modelling/Deterministic_Modeling#E7_production_system previous modeling exercise]. The E7 and immunity protein production has been cut down.
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==Lysis production Sensitivity Analysis==
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== Detection & Lysis production Sensitivity Analysis==
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[[Image:Sensitivity_for_lysis.jpg|800px|thumb|center|Sensitivity analysis for Lysis system]]
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[[Image:Sensitivity_for_lysis.jpg|800px|thumb|center|Sensitivity analysis for Detection & Lysis system]]
The Sensitivity analysis revealed that the degradation rate of Proteins was a major factor and the previous assumption was not that accurate. The value for protein degradation was adjusted by assuming that the half time for protein degradation is 10 minutes. The new Lysis output according to the model is as follows.
The Sensitivity analysis revealed that the degradation rate of Proteins was a major factor and the previous assumption was not that accurate. The value for protein degradation was adjusted by assuming that the half time for protein degradation is 10 minutes. The new Lysis output according to the model is as follows.
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[[Image:Lysis_adjust.jpg|thumb|center|800px|New Lysis system model]]<br>
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[[Image:Lysis_adjust.jpg|thumb|center|800px|Detection & Lysis System Version 1]]<br>
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As we can see, the output of protein has been drastically reduced from the [https://2008.igem.org/Team:NTU-Singapore/Modelling/Deterministic_Modeling#Lysis_Production_system previous modeling exercise] for the Lysis Protein Production system.
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As we can see, the output of protein has been drastically reduced from the [https://2008.igem.org/Team:NTU-Singapore/Modelling/Deterministic_Modeling#Lysis_Production_system previous modeling exercise] for the Detection & Lysis Protein Production system.
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Latest revision as of 12:49, 28 October 2008

Sensitivity Analysis

Sensitivty analyses for the two systems were performed using [http://www.sbtoolbox2.org/main.php SBtoolbox 2]. The reason for performing this analysis is to find out which are the important parameters and would have a major effects on the experimental results. A part of the reason is because some of the parameters were fitted and not obtained strictly from literature. This analysis would allow us to plan for experiments while taking into account the effect these parameters may have.

E7 production system Sensitivity Analysis

Sensitivity Analysis for E7 Production

New E7 production

We can compare the new adjusted E7 production system with the previous modeling exercise. The E7 and immunity protein production has been cut down.

Detection & Lysis production Sensitivity Analysis

Sensitivity analysis for Detection & Lysis system

The Sensitivity analysis revealed that the degradation rate of Proteins was a major factor and the previous assumption was not that accurate. The value for protein degradation was adjusted by assuming that the half time for protein degradation is 10 minutes. The new Lysis output according to the model is as follows.


Detection & Lysis System Version 1

As we can see, the output of protein has been drastically reduced from the previous modeling exercise for the Detection & Lysis Protein Production system.

Retrieved from "http://2008.igem.org/Team:NTU-Singapore/Modelling/Sensitivity_Analysis"