Team:Chiba/Calendar-Home/14 September 2008

From 2008.igem.org

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(→Team:Output)
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Line 5: Line 5:
==Laboratory work==
==Laboratory work==
===Team:Communication===
===Team:Communication===
-
:(13/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
 
 +
(13/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
 +
::*[http://partsregistry.org/Part:BBa_I7106 BBa_I7106]
 +
::*[http://partsregistry.org/Part:BBa_I23019 BBa_I23019]
 +
(13/9)--->[[https://2008.igem.org/Team:Chiba/protocol/ligation/dephosphorylation|SAP]]
 +
::*[http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
 +
::*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
-
:'''[[Team:Chiba/protocol/transformation|Transformation]]'''
+
:--->(19/9)
 +
 
 +
'''[[Team:Chiba/protocol/transformation|Transformation]]'''
:Competent cells : XL10Gold
:Competent cells : XL10Gold
::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)
::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)
Line 15: Line 22:
::*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA)
::*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA)
-
:--->(15/9)
+
:--->(19/9)'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 +
 
 +
 
 +
'''[[Team:Chiba/protocol/Time Delay Test|Time Delay Test]]'''
 +
 
 +
 
Line 31: Line 43:
*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141]
*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141]
*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
-
*BBa_I716542
+
*[http://partsregistry.org/Part:BBa_I716452 BBa_I716452]
*[http://partsregistry.org/Part:BBa_I716462 BBa_I716462]
*[http://partsregistry.org/Part:BBa_I716462 BBa_I716462]
*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078]
*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078]
Line 41: Line 53:
*[http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
*[http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
*[http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
*[http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
-
*[http://partsregistry.org/Part:BBa_I7100 BBa_I7100](x2)
+
*[http://partsregistry.org/Part:BBa_I7100 BBa_I7100](×2)
*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
Line 50: Line 62:
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(digested sample)(μL)</td>
+
<td>DNA(digested sample)(&mu;L)</td>
<td>3</td>
<td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer2(x10)(μL)</td>
+
<td>Buffer2(x10)(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O(μL)</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>6</td>
<td>6</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ(μL)</td>
+
<td>EcoRI(&mu;L)</td>
<td>0.075</td>
<td>0.075</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL(μL)</td>
+
<td>TOTAL(&mu;L)</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
</table>
</table>
-
--> left at37 degrees for 1 hour  
+
--> left at 37&deg;C for 1 hour  
[[Team:Chiba/protocol/gelcheck|Gel check]]
[[Team:Chiba/protocol/gelcheck|Gel check]]
Line 81: Line 93:
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(digestion sample)(μL)</td>
+
<td>DNA(digestion sample)(&mu;L)</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye(μL)</td>
+
<td>Loading Dye(&mu;L)</td>
<td>2</td>
<td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL(μL)</td>
+
<td>TOTAL(&mu;L)</td>
<td>12</td>
<td>12</td>
</tr>
</tr>
</table>
</table>

Latest revision as of 23:59, 29 October 2008

>Home | Notebook

13 September 2008 <|> 15 September 2008

Laboratory work

Team:Communication

(13/9)--->Mini prep

  • [http://partsregistry.org/Part:BBa_I7106 BBa_I7106]
  • [http://partsregistry.org/Part:BBa_I23019 BBa_I23019]

(13/9)--->[[1]]

  • [http://partsregistry.org/Part:BBa_J04500 BBa_J04500]
  • [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
--->(19/9)

Transformation

Competent cells : XL10Gold
  • [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084009 BBa_K084009](Plac+RhlI+LVA)
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA)
--->(19/9)Gel Check


Time Delay Test



Team:Output

Time Responce Liquid

Digestion test

  • [http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510]
  • [http://partsregistry.org/Part:BBa_I716463 BBa_I716463]
  • [http://partsregistry.org/Part:BBa_C0051 BBa_C0051]
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070]
  • [http://partsregistry.org/Part:BBa_J06620 BBa_J06620]
  • [http://partsregistry.org/Part:BBa_J22141 BBa_J22141]
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
  • [http://partsregistry.org/Part:BBa_I716452 BBa_I716452]
  • [http://partsregistry.org/Part:BBa_I716462 BBa_I716462]
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078]
  • [http://partsregistry.org/Part:BBa_J01101 BBa_J01101]
  • [http://partsregistry.org/Part:BBa_C0071 BBa_C0071]
  • [http://partsregistry.org/Part:BBa_I712019 BBa_I712019]
  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
  • [http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
  • [http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
  • [http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
  • [http://partsregistry.org/Part:BBa_I7100 BBa_I7100](×2)
  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
Sample No. each
DNA(digested sample)(μL) 3
Buffer2(x10)(μL) 1
dH2O(μL) 6
EcoRI(μL) 0.075
TOTAL(μL) 10

--> left at 37°C for 1 hour

Gel check

Sample No. each
DNA(digestion sample)(μL) 10
Loading Dye(μL) 2
TOTAL(μL) 12
Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/14_September_2008"