Team:Chiba/Calendar-Home/18 September 2008

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Latest revision as of 00:01, 30 October 2008

>Home | Notebook

17 September 2008 <|> 19 September 2008

Laboratory work

Team:Communication

time-delay test
- transformants
  - [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
  - [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
  - [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
- E.coli strain
  Senders:XL10GOLD
  Receiver:JW1908
- temparature
  37°C and 30°C

Team:Output

SAP 1μL

->left for:

1 hour at 37 °C
30 minutes at 65 °C

Zymoclean

->eluted with 25μL of NFW.

Ligation

vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119]
insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084004 K084004]
insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084005 K084005]
insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084006 K084006]

Sample No.	Vector&insert	Vector
DNA(vector)(μL)	3.5	3.5
DNA(insert)(μL)	7.5	-
Ligase(μL)	1	1
Ligase Buffer(μL)	4	4
dH₂O(μL)	4	4
TOTAL(μL)	20	20

transformation

[http://partsregistry.org/Part:BBa_J54103 BBa_J54103]
[http://partsregistry.org/Part:BBa_P2006 BBa_P2006]

Gel check

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003]
[http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
[http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
[http://partsregistry.org/Part:BBa_J37032 BBa_J37032]

Sample No.	each
DNA(μL)	each
Loading Dye(μL)	1
dH₂O(μL)	4
TOTAL(μL)	6

@@ Line 7: / Line 7: @@
 ===Team:Communication===
 *[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
-**conditions
+**transformants
-#strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
+***[http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
-#temparature<br>37℃ and 30℃
+***[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
+***[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
+**E.coli strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
+**temparature<br>37&deg;C  and 30&deg;C
 ===Team:Output===
-[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]] 1μL
+[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]] 1&mu;L
 ->left for:
-#1 hour at 37 degrees
+#1 hour at 37 &deg;C
-#30 minutes at 65 degrees.
+#30 minutes at 65 &deg;C
 [[Team:Chiba/protocol/transformation|Zymoclean]]
-->eluted with 25μL of NFW.
+->eluted with 25&mu;L of NFW.
 [[Team:Chiba/protocol/ligation/ligation|Ligation]]
 *vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
-*insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119](ptet+LuxR)
+*insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119]
-*Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR)
+*insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084004 K084004]
+*insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084005 K084005]
+*insert:[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084006 K084006]
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
@@ Line 35: / Line 40: @@
 </tr>
 <tr>
-<td>DNA(vector)(μL)</td>
+<td>DNA(vector)(&mu;L)</td>
 <td>3.5</td><td>3.5</td>
 </tr>
 <tr>
-<td>DNA(insert)(μL)</td>
+<td>DNA(insert)(&mu;L)</td>
 <td>7.5</td><td>-</td>
 </tr>
 <tr>
-<td>Ligase(μL)</td>
+<td>Ligase(&mu;L)</td>
 <td>1</td><td>1</td>
 </tr>
 <tr>
-<td>Ligase Buffer(μL)</td>
+<td>Ligase Buffer(&mu;L)</td>
 <td>4</td><td>4</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O(μL)</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>4</td><td>4</td>
 </tr>
 <tr>
-<td>TOTAL(μL)</td>
+<td>TOTAL(&mu;L)</td>
 <td>20</td><td>20</td>
 </tr>
@@ Line 61: / Line 66: @@
 [[Team:Chiba/protocol/transformation|transformation]]
-*[http://partsregistry.org/Part:BBa_J54103 BBa_J54103](RBS+GFP)
+*[http://partsregistry.org/Part:BBa_J54103 BBa_J54103]
 *[http://partsregistry.org/Part:BBa_P2006 BBa_P2006]
 [[Team:Chiba/protocol/gelcheck|Gel check]]
-*Ligation product(Venus YFP)
+*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003]
 *[http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
 *[http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
@@ Line 76: / Line 81: @@
 </tr>
 <tr>
-<td>DNA(μL)</td>
+<td>DNA(&mu;L)</td>
 <td>each</td>
 </tr>
 <tr>
-<td>Loading Dye(μL)</td>
+<td>Loading Dye(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O(μL)</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>4</td>
 </tr>
 <tr>
-<td>TOTAL(μL)</td>
+<td>TOTAL(&mu;L)</td>
 <td>6</td>
 </tr>
 </table>