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Team:Chiba/Calendar-Home/12 September 2008
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*Pbad/araC:L-Arabinose induction test | *Pbad/araC:L-Arabinose induction test | ||
| - | *BBa_I1304 | + | *[http://partsregistry.org/Part:BBa_I1304 BBa_I1304]×1(1)(negative control) |
| - | *BBa_R0051( | + | *[http://partsregistry.org/Part:BBa_R0051 BBa_R0051]×4(1)~(5)Select single colony from Agar Plate and introduce to 2mlLB each other. |
| - | #incubated cultures at 37 | + | |
| + | #incubated cultures at 37 °C; for 12hours. | ||
#Measured OD<sub>600</sub>. | #Measured OD<sub>600</sub>. | ||
| Line 16: | Line 17: | ||
Reaction mixtures were mixed in 48-well plate(deep well). | Reaction mixtures were mixed in 48-well plate(deep well). | ||
| - | + | ||
<table width="257" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="257" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
<td width="257">Sample No.</td> | <td width="257">Sample No.</td> | ||
| - | <td> | + | <td>1</td><td>2</td><td>3</td><td>4</td><td>5</td> |
<tr> | <tr> | ||
| - | <td>Cultures( | + | <td>Cultures(μL)</td> |
| - | <td> | + | <td>0μl</td><td>1.9μl</td><td>19μl</td><td>190μl</td><td>-</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> 20%L-Arabinose( | + | <td> 20%L-Arabinose(μL)</td> |
| - | <td> | + | <td>0μl</td><td>1.9μl</td><td>19μl</td><td>190μl</td><td>-</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 35: | Line 36: | ||
Measured the Green Fluorescence intensity by Fluoroskan Ascent 2.5(program:Ascent Software Version 2.6). | Measured the Green Fluorescence intensity by Fluoroskan Ascent 2.5(program:Ascent Software Version 2.6). | ||
| - | + | Add L-arabinose and 30min,1h,2h,6h,12h,24h later, we measured intensicty of fluorescence. | |
| - | L- | + | |
| - | + | ||
| - | + | ||
Result: | Result: | ||
<table width="400" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="400" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
<td width="2300">Time after arabinose induction / Sample No.</td> | <td width="2300">Time after arabinose induction / Sample No.</td> | ||
| - | <td> | + | <td>1</td><td>2</td><td>3</td><td>4</td><td>5</td> |
<tr> | <tr> | ||
<td>30min</td> | <td>30min</td> | ||
Latest revision as of 21:26, 29 October 2008
11 September 2008 <|> 13 September 2008
Contents |
Laboratory work
Team:Input
- Pbad/araC:L-Arabinose induction test
- [http://partsregistry.org/Part:BBa_I1304 BBa_I1304]×1(1)(negative control)
- [http://partsregistry.org/Part:BBa_R0051 BBa_R0051]×4(1)~(5)Select single colony from Agar Plate and introduce to 2mlLB each other.
- incubated cultures at 37 °C; for 12hours.
- Measured OD600.
Reaction mixtures were mixed in 48-well plate(deep well).
| Sample No. | 1 | 2 | 3 | 4 | 5 |
| Cultures(μL) | 0μl | 1.9μl | 19μl | 190μl | - |
| 20%L-Arabinose(μL) | 0μl | 1.9μl | 19μl | 190μl | - |
| L-Arabinase conc. | 0%(N.C) | 0.2% | 2% | 20% | P.C |
Measured the Green Fluorescence intensity by Fluoroskan Ascent 2.5(program:Ascent Software Version 2.6). Add L-arabinose and 30min,1h,2h,6h,12h,24h later, we measured intensicty of fluorescence. Result:
| Time after arabinose induction / Sample No. | 1 | 2 | 3 | 4 | 5 |
| 30min | 8.890 | 9.752 | 10.10 | 9.413 | 9.701 |
| 1h | 9.161 | 13.93 | 17.37 | 14.97 | 10.60 |
| 2h | 9.417 | 27.96 | 40.25 | 29.87 | 10.72 |
| 6h | 9.933 | 74.99 | 150.9 | 125.0 | 10.04 |
| 12h | 10.64 | 99.39 | 202.88 | 72.08 | 11.76 |
| 24h | 14.66 | 105.6 | 447.3 | 33.31 | 17.11 |
Team:Communication
no work
Team:Output
no work
