Wiki/Team:Warsaw/vectors/pMPM-T5-omega

From 2008.igem.org

(Difference between revisions)
 
Line 5: Line 5:
<h2>pMPM-T5-omega vector</h2>
<h2>pMPM-T5-omega vector</h2>
-
<b>Description</b><br>
+
<p class=vector>Description</p><br>
<p>This vector was a kind gift from the:<br> Department of Virology,<br> Faculty of Biology, <br>University of Warsaw</p><br>
<p>This vector was a kind gift from the:<br> Department of Virology,<br> Faculty of Biology, <br>University of Warsaw</p><br>
-
<p>Using this vector as a backbone we have prepared all of plasmids in the "One-armed bandit" part of the project.</p>
+
<b>Resistance</b>: tetracycline<br>
-
 
+
<b>Features</b>: Inducible arabinose promoter<br>
 +
<b>Usage</b>: Using this vector as a backbone we have prepared all of plasmids in the "One-armed bandit" part of the project
 +
<br><br>
 +
<p class=vector>Vector map</p><br><br>
<img src="https://static.igem.org/mediawiki/2008/3/3d/PMPM-T5-omega.png" width=600>
<img src="https://static.igem.org/mediawiki/2008/3/3d/PMPM-T5-omega.png" width=600>
<br><br>
<br><br>
-
vector sequence:
+
<p class=vector>Vector sequence</p><br><br>
<pre>
<pre>

Latest revision as of 23:02, 26 October 2008

Gallery Bricks Notebook Team Project Home


Back to previous page
Vectors view

 

pMPM-T5-omega vector

Description


This vector was a kind gift from the:
Department of Virology,
Faculty of Biology,
University of Warsaw


Resistance: tetracycline
Features: Inducible arabinose promoter
Usage: Using this vector as a backbone we have prepared all of plasmids in the "One-armed bandit" part of the project

Vector map





Vector sequence



ATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAAC
CAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAG
ATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAAC
GTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCA
CTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAA
GCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGA
AAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGC
GCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCC
GCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTG
CGCAACTGTTGGGAAGGGCGCGGGCCATTATTGAAGCATTTATCAGGGTTA
TTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAAA
GAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGACTGTCTGCTTAATTTG
ATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTG
CTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGC
GTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGC
CTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGAAGACC
CACACTACCATCGGGCCTACGGGCTTTCACTTCTAGTTGGGCATGGGGTCA
GGTGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACGCT
TATGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCC
AAAACAGCCAAGCTGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTGAT
ATCGCATGCCTGCAGCCCGGGAGATCTACTAGTTCTAGagctttatgcttg
taaaccgttttgtgaaaaaatttttaaaataaaaaaggggacctctagggt
ccccaattaattagtaatataatctattaaaggtcattcaaaaggtcatcc
accggatcagcttagtaaagccctcgctagattttaatgcggatgttgcga
ttacttcgccaactattgcgataacaagaaaaagccagcctttcatgatat
atctcccaatttgtgtagggcttattatgcacgcttaaaaataataaaagc
agacttgacctgatagtttggctgtgagcaattatgtgcttagtgcatcta
acgcttgagttaagccgcgccgcgaagcggcgtcggcttgaacgaattgtt
agacattatttgccgactaccttggtgatctcgcctttcacgtagtggaca
aattcttccaactgatctgcgcgcgaggccaagcgatcttcttcttgtcca
agataagcctgtctagcttcaagtatgacgggctgatactgggccggcagg
cgctccattgcccagtcggcagcgacatccttcggcgcgattttgccggtt
actgcgctgtaccaaatgcgggacaacgtaagcactacatttcgctcatcg
ccagcccagtcgggcggcgagttccatagcgttaaggtttcatttagcgcc
tcaaatagatcctgttcaggaaccggatcaaagagttcctccgccgctgga
cctaccaaggcaacgctatgttctcttgcttttgtcagcaagatagccaga
tcaatgtcgatcgtggctggctcgaagatacctgcaagaatgtcattgcgc
tgccattctccaaattgcagttcgcgcttagctggataacgccacggaatg
atgtcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcgctc
tctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgc
gttgtttcatcaagccttacggtcaccgtaaccagcaaatcaatatcactg
tgtggcttcaggccgccatccactgcggagccgtacaaatgtacggccagc
aacgtcggttcgagatggcgctcgatgacgccaactacctctgatagttga
gtcgatacttcggcgatcaccgcttccctcatgatgtttaactttgtttta
gggcgactgccctgctgcgtaacatcgttgctgctccataacatcaaacat
cgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgt
accccaaaaaaacagtcataacaagccatgaaaaccgccactgcgccgtta
ccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgc
tacttgcattacagcttacgaaccgaacaggcttatgtccactgggttcgt
gccttcatccgtttccacggtgtgcgtcacccggcaaccttgggcagcagc
gaagtcgaggcatttctgtcctggctggcgaacgagcgcaaggtttcggtc
tccacgcatcgtcaggcattggcggccttgctgttcttctacggcaaggtg
ctgtgcacggatctgccctggcttcaggagatcggaagacctcggccgtcg
cggcgcttgccggtggtgctgaccccggatgaagtggttcgcatcctcggt
tttctggaaggcgagcatcgtttgttcgcccagcttctgtatggaacgggc
atgcggatcagtgagggtttgcaactgcgggtcaaggatctggatttcgat
cacggcacgatcatcgtgcgggagggcaagggctccaaggatcgggccttg
atgttacccgagagcttggcacccagcctgcgcgagcaggggaattgatcc
ggtggatgaccttttgaatgacctttaatagattatattactaattaattg
gggaccctagaggtccccttttttattttaaaaattttttcacaaaacggt
ttacaagcataaagctCTAGAGCCATGGTGAATTCCTCCTTTCACTCCATC
CAAAAAAACGGGTATGGAGAAACAGTAGAGAGTTGCGATAAAAAGCGTCAG
GTAGGATCCGCTAATCTTATGGATAAAAATGCTATGGCATAGCAAAGTGTG
ACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTT
TTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTT
TTAATAAGCGGGGTTACCGGTTGGGTTAGCGAGAAGAGCCAGTAAAAGACG
CAGTGACGGCAATGTCTGATGCAATATGGACAATTGGTTTCTTCTCTGAAT
GGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCG
GGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCC
AACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTAT
ATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGA
GAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATT
CATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTT
TACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATA
TTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTC
AGCGACCTGTTTGGGCAAATCATTAACGCCGGGCAAGGGGAAGGGCGCTAT
TCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGTTACTGCGGCGCATG
GAAGCGATTAACGAGTCGCTCCATCCGCCGATGGATAATCGGGTACGCGAG
GCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCC
AGCGTCGCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTC
CGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATC
AGCCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTC
GGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTATTTAAA
AAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGTGAAGAAAAA
GTGAATGATGTAGCCGTCAAGTTGTCATAATTGGTAACGAATCAGACAATT
GACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATT
TGACAGGCCACATTATGCAAGCATTGCGGTAATGAATCGGCCAACGCGCGG
GGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACT
CGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGG
CATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAAC
GAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCA
TACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATC
ATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTAC
CCCCATGAACAGAAATTCCCCCTTACACGGAGGCATCAAGTGACCAAACAG
GAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACG
CTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGT
GAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGT
TTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTC
ACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCG
TCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGC
GATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTAC
TGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAG
AAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCG
CTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAAT
ACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAA
AGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTT
CCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCA
GAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGG
AAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCT
GTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTG
TAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCA
CGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCT
TGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGG
TAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAA
GTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGC
TCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGG
CAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGAT
TACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGG
GTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGCC
GAGATGCGCCGCGTGCGGCTGCTGGAGATGGCGGACGCGATGGATATGTTC
TGCCAAGGGTTGGTTTGCGCATTCACAGTTCTCCGCAAGAATTGATTGGCT
CCAATTCTTGGAGTGGTGAATCCGTTAGCGAGGTGCCGCCGGCTTCCATTC
AGGTCGAGGTGGCCCGGCTCCATGCACCGCGACGCAACGCGGGGAGGCAGA
CAAGGTATAGGGCGGCGCCTACAATCCATGCCAACCCGTTCCATGTGCTCG
CCGAGGCGGCATAAATCGCCGTGACGATCAGCGGTCCAGTGATCGAAGTTA
GGCTGGTAAGAGCCGCGAGCGATCCTTGAAGCTGTCCCTGATGGTCGTCAT
CTACCTGCCTGGACAGCATGGCCTGCAACGCGGGCATCCCGATGCCGCCGG
AAGCGAGAAGAATCATAATGGGGAAGGCCATCCAGCCTCGCGTCGCGAACG
CCAGCAAGACGTAGCCCAGCGCGTCGGCCGCCATGCCGGCGATAATGGCCT
GCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGA
GGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGC
TCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCT
GTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAG
TCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGG
GCATCGGTCGGCGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCA
GTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGTA
AGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCA
CGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCAT
CGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGA
TGCCGGCCACGATGCGTCCGGCGTAGAGAATCCACAGGACGGGTGTGGTCG
CCATGATCGCGTAGTCGATAGTGGCTCCAAGTAGCGAAGCGAGCAGGACTG
GGCGGCGGCCAAAGCGGTCGGACAGTGCTCCGAGAACGGGTGCGCATAGAA
ATTGCATCAACGCATATAGCGCTAGCAGCACGCCATAGTGACTGGCGATGC
TGTCGGAATGGACGATATCCCGCAAGAGGCCCGGCAGTACCGGCATAACCA
AGCCTATGCCTACAGCATCCAGGGTGACGGTGCCGAGGATGACGATGAGCG
CATTGTTAGATTTCATACACGGTGCCTGACTGCGTTAGCAATTTAACTGTG
ATAAACTACCGCATTAAAGCTAATCGATGATAAGCTGTCAAACATGAGAAT
T


Retrieved from "http://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega"